1y0q
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1y0q.gif|left|200px]] | + | [[Image:1y0q.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of an active group I ribozyme-product complex''' | + | {{Structure |
| + | |PDB= 1y0q |SIZE=350|CAPTION= <scene name='initialview01'>1y0q</scene>, resolution 3.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SPK:SPERMINE+(FULLY+PROTONATED+FORM)'>SPK</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= orf142-I2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=55510 Staphylococcus phage Twort]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of an active group I ribozyme-product complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1Y0Q is a [ | + | 1Y0Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_phage_twort Staphylococcus phage twort]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y0Q OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:[http:// | + | Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15580277 15580277] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Staphylococcus phage twort]] | [[Category: Staphylococcus phage twort]] | ||
| Line 24: | Line 33: | ||
[[Category: rna-splicing]] | [[Category: rna-splicing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:17:00 2008'' |
Revision as of 13:17, 20 March 2008
| |||||||
| , resolution 3.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | orf142-I2 (Staphylococcus phage Twort) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of an active group I ribozyme-product complex
Overview
Group I introns are catalytic RNAs capable of orchestrating two sequential phosphotransesterification reactions that result in self-splicing. To understand how the group I intron active site facilitates catalysis, we have solved the structure of an active ribozyme derived from the orf142-I2 intron from phage Twort bound to a four-nucleotide product RNA at a resolution of 3.6 A. In addition to the three conserved domains characteristic of all group I introns, the Twort ribozyme has peripheral insertions characteristic of phage introns. These elements form a ring that completely envelops the active site, where a snug pocket for guanosine is formed by a series of stacked base triples. The structure of the active site reveals three potential binding sites for catalytic metals, and invokes a role for the 2' hydroxyl of the guanosine substrate in organization of the active site for catalysis.
About this Structure
1Y0Q is a Protein complex structure of sequences from Staphylococcus phage twort. Full crystallographic information is available from OCA.
Reference
Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:15580277
Page seeded by OCA on Thu Mar 20 15:17:00 2008
Categories: Protein complex | Staphylococcus phage twort | Chase, E. | Golden, B L. | Kim, H. | MG | SO4 | SPK | Guanosine-binding | Ribozyme | Rna-folding | Rna-splicing
