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1y0q

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[[Image:1y0q.gif|left|200px]]<br /><applet load="1y0q" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1y0q.gif|left|200px]]
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caption="1y0q, resolution 3.60&Aring;" />
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'''Crystal structure of an active group I ribozyme-product complex'''<br />
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{{Structure
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|PDB= 1y0q |SIZE=350|CAPTION= <scene name='initialview01'>1y0q</scene>, resolution 3.60&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SPK:SPERMINE+(FULLY+PROTONATED+FORM)'>SPK</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY=
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|GENE= orf142-I2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=55510 Staphylococcus phage Twort])
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}}
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'''Crystal structure of an active group I ribozyme-product complex'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1Y0Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_phage_twort Staphylococcus phage twort] with <scene name='pdbligand=SPK:'>SPK</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y0Q OCA].
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1Y0Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_phage_twort Staphylococcus phage twort]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y0Q OCA].
==Reference==
==Reference==
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Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15580277 15580277]
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Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15580277 15580277]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Staphylococcus phage twort]]
[[Category: Staphylococcus phage twort]]
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[[Category: rna-splicing]]
[[Category: rna-splicing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:00:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:17:00 2008''

Revision as of 13:17, 20 March 2008

Image:1y0q.gif


PDB ID 1y0q

Drag the structure with the mouse to rotate
, resolution 3.60Å
Ligands: , and
Gene: orf142-I2 (Staphylococcus phage Twort)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of an active group I ribozyme-product complex


Overview

Group I introns are catalytic RNAs capable of orchestrating two sequential phosphotransesterification reactions that result in self-splicing. To understand how the group I intron active site facilitates catalysis, we have solved the structure of an active ribozyme derived from the orf142-I2 intron from phage Twort bound to a four-nucleotide product RNA at a resolution of 3.6 A. In addition to the three conserved domains characteristic of all group I introns, the Twort ribozyme has peripheral insertions characteristic of phage introns. These elements form a ring that completely envelops the active site, where a snug pocket for guanosine is formed by a series of stacked base triples. The structure of the active site reveals three potential binding sites for catalytic metals, and invokes a role for the 2' hydroxyl of the guanosine substrate in organization of the active site for catalysis.

About this Structure

1Y0Q is a Protein complex structure of sequences from Staphylococcus phage twort. Full crystallographic information is available from OCA.

Reference

Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:15580277

Page seeded by OCA on Thu Mar 20 15:17:00 2008

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