1y18
From Proteopedia
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| - | [[Image:1y18.gif|left|200px]] | + | [[Image:1y18.gif|left|200px]] |
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| - | '''Fab fragment of catalytic elimination antibody 34E4 E(H50)D mutant in complex with hapten''' | + | {{Structure |
| + | |PDB= 1y18 |SIZE=350|CAPTION= <scene name='initialview01'>1y18</scene>, resolution 2.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=HAN:2-AMINO-5,6-DIMETHYL-BENZIMIDAZOLE-1-PENTANOIC ACID'>HAN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Fab fragment of catalytic elimination antibody 34E4 E(H50)D mutant in complex with hapten''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Y18 is a [ | + | 1Y18 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus,_homo_sapiens Mus musculus, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y18 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural origins of efficient proton abstraction from carbon by a catalytic antibody., Debler EW, Ito S, Seebeck FP, Heine A, Hilvert D, Wilson IA, Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):4984-9. Epub 2005 Mar 23. PMID:[http:// | + | Structural origins of efficient proton abstraction from carbon by a catalytic antibody., Debler EW, Ito S, Seebeck FP, Heine A, Hilvert D, Wilson IA, Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):4984-9. Epub 2005 Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15788533 15788533] |
[[Category: Mus musculus, homo sapiens]] | [[Category: Mus musculus, homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:17:10 2008'' |
Revision as of 13:17, 20 March 2008
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| , resolution 2.80Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Fab fragment of catalytic elimination antibody 34E4 E(H50)D mutant in complex with hapten
Overview
Antibody 34E4 catalyzes the conversion of benzisoxazoles to salicylonitriles with high rates and multiple turnovers. The crystal structure of its complex with the benzimidazolium hapten at 2.5-angstroms resolution shows that a combination of hydrogen bonding, pi stacking, and van der Waals interactions is exploited to position both the base, Glu(H50), and the substrate for efficient proton transfer. Suboptimal placement of the catalytic carboxylate, as observed in the 2.8-angstroms structure of the Glu(H50)Asp variant, results in substantially reduced catalytic efficiency. In addition to imposing high positional order on the transition state, the antibody pocket provides a highly structured microenvironment for the reaction in which the carboxylate base is activated through partial desolvation, and the highly polarizable transition state is stabilized by dispersion interactions with the aromatic residue Trp(L91) and solvation of the leaving group oxygen by external water. The enzyme-like efficiency of general base catalysis in this system directly reflects the original hapten design, in which a charged guanidinium moiety was strategically used to elicit an accurately positioned functional group in an appropriate reaction environment and suggests that even larger catalytic effects may be achievable by extending this approach to the induction of acid-base pairs capable of bifunctional catalysis.
About this Structure
1Y18 is a Protein complex structure of sequences from Mus musculus, homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural origins of efficient proton abstraction from carbon by a catalytic antibody., Debler EW, Ito S, Seebeck FP, Heine A, Hilvert D, Wilson IA, Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):4984-9. Epub 2005 Mar 23. PMID:15788533
Page seeded by OCA on Thu Mar 20 15:17:10 2008
