1y27
From Proteopedia
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| - | [[Image:1y27.gif|left|200px]] | + | [[Image:1y27.gif|left|200px]] |
| - | + | ||
| - | '''G-riboswitch-guanine complex''' | + | {{Structure |
| + | |PDB= 1y27 |SIZE=350|CAPTION= <scene name='initialview01'>1y27</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO2:HYPOPHOSPHITE'>PO2</scene> and <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= XPT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | }} | ||
| + | |||
| + | '''G-riboswitch-guanine complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Y27 is a [ | + | 1Y27 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y27 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs., Serganov A, Yuan YR, Pikovskaya O, Polonskaia A, Malinina L, Phan AT, Hobartner C, Micura R, Breaker RR, Patel DJ, Chem Biol. 2004 Dec;11(12):1729-41. PMID:[http:// | + | Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs., Serganov A, Yuan YR, Pikovskaya O, Polonskaia A, Malinina L, Phan AT, Hobartner C, Micura R, Breaker RR, Patel DJ, Chem Biol. 2004 Dec;11(12):1729-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15610857 15610857] |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: g-riboswitch guanine recognition]] | [[Category: g-riboswitch guanine recognition]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:17:32 2008'' |
Revision as of 13:17, 20 March 2008
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| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | XPT (Bacillus subtilis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
G-riboswitch-guanine complex
Overview
Metabolite-sensing mRNAs, or "riboswitches," specifically interact with small ligands and direct expression of the genes involved in their metabolism. Riboswitches contain sensing "aptamer" modules, capable of ligand-induced structural changes, and downstream regions, harboring expression-controlling elements. We report the crystal structures of the add A-riboswitch and xpt G-riboswitch aptamer modules that distinguish between bound adenine and guanine with exquisite specificity and modulate expression of two different sets of genes. The riboswitches form tuning fork-like architectures, in which the prongs are held in parallel through hairpin loop interactions, and the internal bubble zippers up to form the purine binding pocket. The bound purines are held by hydrogen bonding interactions involving conserved nucleotides along their entire periphery. Recognition specificity is associated with Watson-Crick pairing of the encapsulated adenine and guanine ligands with uridine and cytosine, respectively.
About this Structure
1Y27 is a Protein complex structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs., Serganov A, Yuan YR, Pikovskaya O, Polonskaia A, Malinina L, Phan AT, Hobartner C, Micura R, Breaker RR, Patel DJ, Chem Biol. 2004 Dec;11(12):1729-41. PMID:15610857
Page seeded by OCA on Thu Mar 20 15:17:32 2008
