1y42
From Proteopedia
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| - | [[Image:1y42.gif|left|200px]] | + | [[Image:1y42.gif|left|200px]] |
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| - | '''Crystal structure of a C-terminally truncated CYT-18 protein''' | + | {{Structure |
| + | |PDB= 1y42 |SIZE=350|CAPTION= <scene name='initialview01'>1y42</scene>, resolution 1.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=TYR:TYROSINE'>TYR</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] | ||
| + | |GENE= cyt-18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5141 Neurospora crassa]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a C-terminally truncated CYT-18 protein''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Y42 is a [ | + | 1Y42 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y42 OCA]. |
==Reference== | ==Reference== | ||
| - | A tyrosyl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface., Paukstelis PJ, Coon R, Madabusi L, Nowakowski J, Monzingo A, Robertus J, Lambowitz AM, Mol Cell. 2005 Feb 4;17(3):417-28. PMID:[http:// | + | A tyrosyl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface., Paukstelis PJ, Coon R, Madabusi L, Nowakowski J, Monzingo A, Robertus J, Lambowitz AM, Mol Cell. 2005 Feb 4;17(3):417-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15694342 15694342] |
[[Category: Neurospora crassa]] | [[Category: Neurospora crassa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tyrosyl trna synthetase]] | [[Category: tyrosyl trna synthetase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:18:09 2008'' |
Revision as of 13:18, 20 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | cyt-18 (Neurospora crassa) | ||||||
| Activity: | Tyrosine--tRNA ligase, with EC number 6.1.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a C-terminally truncated CYT-18 protein
Overview
We determined a 1.95 A X-ray crystal structure of a C-terminally truncated Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) that functions in splicing group I introns. CYT-18's nucleotide binding fold and intermediate alpha-helical domains superimpose on those of bacterial TyrRSs, except for an N-terminal extension and two small insertions not found in nonsplicing bacterial enzymes. These additions surround the cyt-18-1 mutation site and are sites of suppressor mutations that restore splicing, but not synthetase activity. Highly constrained models based on directed hydroxyl radical cleavage assays show that the group I intron binds at a site formed in part by the three additions on the nucleotide binding fold surface opposite that which binds tRNATyr. Our results show how essential proteins can progressively evolve new functions.
About this Structure
1Y42 is a Single protein structure of sequence from Neurospora crassa. Full crystallographic information is available from OCA.
Reference
A tyrosyl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface., Paukstelis PJ, Coon R, Madabusi L, Nowakowski J, Monzingo A, Robertus J, Lambowitz AM, Mol Cell. 2005 Feb 4;17(3):417-28. PMID:15694342
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