1y47
From Proteopedia
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| - | [[Image:1y47.gif|left|200px]] | + | [[Image:1y47.gif|left|200px]] |
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| - | '''Structural studies of designed alpha-helical hairpins''' | + | {{Structure |
| + | |PDB= 1y47 |SIZE=350|CAPTION= <scene name='initialview01'>1y47</scene>, resolution 2.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CD:CADMIUM ION'>CD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structural studies of designed alpha-helical hairpins''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Y47 is a [ | + | 1Y47 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y47 OCA]. |
==Reference== | ==Reference== | ||
| - | Analysis and design of turns in alpha-helical hairpins., Lahr SJ, Engel DE, Stayrook SE, Maglio O, North B, Geremia S, Lombardi A, DeGrado WF, J Mol Biol. 2005 Mar 11;346(5):1441-54. Epub 2005 Jan 13. PMID:[http:// | + | Analysis and design of turns in alpha-helical hairpins., Lahr SJ, Engel DE, Stayrook SE, Maglio O, North B, Geremia S, Lombardi A, DeGrado WF, J Mol Biol. 2005 Mar 11;346(5):1441-54. Epub 2005 Jan 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713492 15713492] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: DeGrado, W F.]] | [[Category: DeGrado, W F.]] | ||
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[[Category: Stayrook, S E.]] | [[Category: Stayrook, S E.]] | ||
[[Category: CD]] | [[Category: CD]] | ||
| - | [[Category: diiron | + | [[Category: diiron protein]] |
[[Category: helical hairpin]] | [[Category: helical hairpin]] | ||
[[Category: protein design]] | [[Category: protein design]] | ||
| - | [[Category: | + | [[Category: turn]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:18:11 2008'' |
Revision as of 13:18, 20 March 2008
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| , resolution 2.70Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural studies of designed alpha-helical hairpins
Overview
Although the analysis and design of turns that connect the strands in antiparallel beta-hairpins has reached an advanced state, much less is known concerning turns between antiparallel helices in helical hairpins. We have conducted an analysis of the structures and sequence preferences of two types of interhelical turns, each of which connects the two helices by a two-residue linker in an alphaL-beta conformation. Based on this analysis, it became apparent that the turn introduced into a designed four-helix bundle protein, DF1, did not occur within an optimal structural context. DF1 is a dimeric model for the diiron class of proteins. A longer loop with a beta-alphaR-beta conformation was inserted between two helices in the protein, and a sequence was chosen to stabilize its conformation. X-ray crystallography and NMR analysis of the protein showed the structure to be in excellent agreement with design.
About this Structure
1Y47 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Analysis and design of turns in alpha-helical hairpins., Lahr SJ, Engel DE, Stayrook SE, Maglio O, North B, Geremia S, Lombardi A, DeGrado WF, J Mol Biol. 2005 Mar 11;346(5):1441-54. Epub 2005 Jan 13. PMID:15713492
Page seeded by OCA on Thu Mar 20 15:18:11 2008
Categories: Protein complex | DeGrado, W F. | Engel, D E. | Geremia, S. | Lahr, S J. | Lombardi, A. | Maglio, O. | North, B. | Stayrook, S E. | CD | Diiron protein | Helical hairpin | Protein design | Turn
