1y62

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[[Image:1y62.gif|left|200px]]<br /><applet load="1y62" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1y62.gif|left|200px]]
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caption="1y62, resolution 2.45&Aring;" />
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'''A 2.4 crystal structure of conkunitzin-S1, a novel Kunitz-fold cone snail neurotoxin.'''<br />
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{{Structure
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|PDB= 1y62 |SIZE=350|CAPTION= <scene name='initialview01'>1y62</scene>, resolution 2.45&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''A 2.4 crystal structure of conkunitzin-S1, a novel Kunitz-fold cone snail neurotoxin.'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1Y62 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y62 OCA].
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1Y62 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y62 OCA].
==Reference==
==Reference==
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Structure of conkunitzin-S1, a neurotoxin and Kunitz-fold disulfide variant from cone snail., Dy CY, Buczek P, Imperial JS, Bulaj G, Horvath MP, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):980-90. Epub 2006, Aug 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16929098 16929098]
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Structure of conkunitzin-S1, a neurotoxin and Kunitz-fold disulfide variant from cone snail., Dy CY, Buczek P, Imperial JS, Bulaj G, Horvath MP, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):980-90. Epub 2006, Aug 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16929098 16929098]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Buczek, P.]]
[[Category: Buczek, P.]]
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[[Category: kunitz fold]]
[[Category: kunitz fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:02:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:18:52 2008''

Revision as of 13:18, 20 March 2008

Image:1y62.gif


PDB ID 1y62

Drag the structure with the mouse to rotate
, resolution 2.45Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



A 2.4 crystal structure of conkunitzin-S1, a novel Kunitz-fold cone snail neurotoxin.


Overview

Cone snails (Conus) are predatory marine mollusks that immobilize prey with venom containing 50-200 neurotoxic polypeptides. Most of these polypeptides are small disulfide-rich conotoxins that can be classified into families according to their respective ion-channel targets and patterns of cysteine-cysteine disulfides. Conkunitzin-S1, a potassium-channel pore-blocking toxin isolated from C. striatus venom, is a member of a newly defined conotoxin family with sequence homology to Kunitz-fold proteins such as alpha-dendrotoxin and bovine pancreatic trypsin inhibitor (BPTI). While conkunitzin-S1 and alpha-dendrotoxin are 42% identical in amino-acid sequence, conkunitzin-S1 has only four of the six cysteines normally found in Kunitz proteins. Here, the crystal structure of conkunitzin-S1 is reported. Conkunitzin-S1 adopts the canonical 3(10)-beta-beta-alpha Kunitz fold complete with additional distinguishing structural features including two completely buried water molecules. The crystal structure, although completely consistent with previously reported NMR distance restraints, provides a greater degree of precision for atomic coordinates, especially for S atoms and buried solvent molecules. The region normally cross-linked by cysteines II and IV in other Kunitz proteins retains a network of hydrogen bonds and van der Waals interactions comparable to those found in alpha-dendrotoxin and BPTI. In conkunitzin-S1, glycine occupies the sequence position normally reserved for cysteine II and the special steric properties of glycine allow additional van der Waals contacts with the glutamine residue substituting for cysteine IV. Evolution has thus defrayed the cost of losing a disulfide bond by augmenting and optimizing weaker yet nonetheless effective non-covalent interactions.

About this Structure

1Y62 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structure of conkunitzin-S1, a neurotoxin and Kunitz-fold disulfide variant from cone snail., Dy CY, Buczek P, Imperial JS, Bulaj G, Horvath MP, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):980-90. Epub 2006, Aug 19. PMID:16929098

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