1y6i
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1y6i.gif|left|200px]] | + | [[Image:1y6i.gif|left|200px]] |
| - | + | ||
| - | '''Synechocystis GUN4''' | + | {{Structure |
| + | |PDB= 1y6i |SIZE=350|CAPTION= <scene name='initialview01'>1y6i</scene>, resolution 1.78Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= gun4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1143 Synechocystis sp.]) | ||
| + | }} | ||
| + | |||
| + | '''Synechocystis GUN4''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1Y6I is a [ | + | 1Y6I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y6I OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the Mg-chelatase cofactor GUN4 reveals a novel hand-shaped fold for porphyrin binding., Verdecia MA, Larkin RM, Ferrer JL, Riek R, Chory J, Noel JP, PLoS Biol. 2005 May;3(5):e151. Epub 2005 Apr 26. PMID:[http:// | + | Structure of the Mg-chelatase cofactor GUN4 reveals a novel hand-shaped fold for porphyrin binding., Verdecia MA, Larkin RM, Ferrer JL, Riek R, Chory J, Noel JP, PLoS Biol. 2005 May;3(5):e151. Epub 2005 Apr 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15884974 15884974] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Synechocystis sp.]] | [[Category: Synechocystis sp.]] | ||
| Line 22: | Line 31: | ||
[[Category: porphyrin binding]] | [[Category: porphyrin binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:18:58 2008'' |
Revision as of 13:18, 20 March 2008
| |||||||
| , resolution 1.78Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | gun4 (Synechocystis sp.) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Synechocystis GUN4
Overview
In plants, the accumulation of the chlorophyll precursor Mg-protoporphyrin IX (Mg-Proto) in the plastid regulates the expression of a number of nuclear genes with functions related to photosynthesis. Analysis of the plastid-to-nucleus signaling activity of Mg-Proto in Arabidopsis thaliana led to the discovery of GUN4, a novel porphyrin-binding protein that also dramatically enhances the activity of Mg-chelatase, the enzyme that synthesizes Mg-Proto. GUN4 may also play a role in both photoprotection and the cellular shuttling of tetrapyrroles. Here we report a 1.78-A resolution crystal structure of Synechocystis GUN4, in which the porphyrin-binding domain adopts a unique three dimensional fold with a "cupped hand" shape. Biophysical and biochemical analyses revealed the specific site of interaction between GUN4 and Mg-Proto and the energetic determinants for the GUN4.Mg-Proto interaction. Our data support a novel protective function for GUN4 in tetrapyrrole trafficking. The combined structural and energetic analyses presented herein form the physical-chemical basis for understanding GUN4 biological activity, including its role in the stimulation of Mg-chelatase activity, as well as in Mg-Proto retrograde signaling.
About this Structure
1Y6I is a Single protein structure of sequence from Synechocystis sp.. Full crystallographic information is available from OCA.
Reference
Structure of the Mg-chelatase cofactor GUN4 reveals a novel hand-shaped fold for porphyrin binding., Verdecia MA, Larkin RM, Ferrer JL, Riek R, Chory J, Noel JP, PLoS Biol. 2005 May;3(5):e151. Epub 2005 Apr 26. PMID:15884974
Page seeded by OCA on Thu Mar 20 15:18:58 2008
