1y8x
From Proteopedia
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| - | [[Image:1y8x.gif|left|200px]] | + | [[Image:1y8x.gif|left|200px]] |
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| - | '''Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1''' | + | {{Structure |
| + | |PDB= 1y8x |SIZE=350|CAPTION= <scene name='initialview01'>1y8x</scene>, resolution 2.4Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] | ||
| + | |GENE= UBE2M, UBC12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), UBE1C, UBA3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Y8X is a [ | + | 1Y8X is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y8X OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1., Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA, Mol Cell. 2005 Feb 4;17(3):341-50. PMID:[http:// | + | Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1., Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA, Mol Cell. 2005 Feb 4;17(3):341-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15694336 15694336] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: Zhuang, M.]] | [[Category: Zhuang, M.]] | ||
[[Category: ligase]] | [[Category: ligase]] | ||
| - | [[Category: ubiquitin-conjugating enzyme e2 m ]] | + | [[Category: ubiquitin-conjugating enzyme e2 m]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:19:50 2008'' |
Revision as of 13:19, 20 March 2008
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| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | UBE2M, UBC12 (Homo sapiens), UBE1C, UBA3 (Homo sapiens) | ||||||
| Activity: | Ubiquitin--protein ligase, with EC number 6.3.2.19 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural basis for recruitment of Ubc12 by an E2-binding domain in NEDD8's E1
Overview
E2 conjugating enzymes play a central role in ubiquitin and ubiquitin-like protein (ublp) transfer cascades: the E2 accepts the ublp from the E1 enzyme and then the E2 often interacts with an E3 enzyme to promote ublp transfer to the target. We report here the crystal structure of a complex between the C-terminal domain from NEDD8's heterodimeric E1 (APPBP1-UBA3) and the catalytic core domain of NEDD8's E2 (Ubc12). The structure and associated mutational analyses reveal molecular details of Ubc12 recruitment by NEDD8's E1. Interestingly, the E1's Ubc12 binding domain resembles ubiquitin and recruits Ubc12 in a manner mimicking ubiquitin's interactions with ubiquitin binding domains. Structural comparison with E2-E3 complexes indicates that the E1 and E3 binding sites on Ubc12 may overlap and raises the possibility that crosstalk between E1 and E3 interacting with an E2 could influence the specificity and processivity of ublp transfer.
About this Structure
1Y8X is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1., Huang DT, Paydar A, Zhuang M, Waddell MB, Holton JM, Schulman BA, Mol Cell. 2005 Feb 4;17(3):341-50. PMID:15694336
Page seeded by OCA on Thu Mar 20 15:19:50 2008
