1ya7
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1ya7.gif|left|200px]] | + | [[Image:1ya7.gif|left|200px]] |
| - | + | ||
| - | '''Implications for interactions of proteasome with PAN and PA700 from the 1.9 A structure of a proteasome-11S activator complex''' | + | {{Structure |
| + | |PDB= 1ya7 |SIZE=350|CAPTION= <scene name='initialview01'>1ya7</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] | ||
| + | |GENE= psmA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2303 Thermoplasma acidophilum]), psmB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2303 Thermoplasma acidophilum]) | ||
| + | }} | ||
| + | |||
| + | '''Implications for interactions of proteasome with PAN and PA700 from the 1.9 A structure of a proteasome-11S activator complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1YA7 is a [ | + | 1YA7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum] and [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YA7 OCA]. |
==Reference== | ==Reference== | ||
| - | The 1.9 A structure of a proteasome-11S activator complex and implications for proteasome-PAN/PA700 interactions., Forster A, Masters EI, Whitby FG, Robinson H, Hill CP, Mol Cell. 2005 May 27;18(5):589-99. PMID:[http:// | + | The 1.9 A structure of a proteasome-11S activator complex and implications for proteasome-PAN/PA700 interactions., Forster A, Masters EI, Whitby FG, Robinson H, Hill CP, Mol Cell. 2005 May 27;18(5):589-99. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15916965 15916965] |
[[Category: Proteasome endopeptidase complex]] | [[Category: Proteasome endopeptidase complex]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 27: | Line 36: | ||
[[Category: pa26]] | [[Category: pa26]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:20:15 2008'' |
Revision as of 13:20, 20 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | psmA (Thermoplasma acidophilum), psmB (Thermoplasma acidophilum) | ||||||
| Activity: | Proteasome endopeptidase complex, with EC number 3.4.25.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Implications for interactions of proteasome with PAN and PA700 from the 1.9 A structure of a proteasome-11S activator complex
Overview
Proteasomes are cylindrical structures that function in multiple cellular processes by degrading a wide variety of cytosolic and nuclear proteins. Substrate access and product release from the enclosed catalytic chamber occurs through axial pores that are opened by activator complexes. Here, we report high-resolution structures of wild-type and mutant archaeal proteasomes bound to the activator PA26. These structures support the proposal that an ordered open conformation is required for proteolysis and that its formation can be triggered by outward displacement of surrounding residues. The structures and associated biochemical assays reveal the mechanism of binding, which involves an interaction between the PA26 C terminus and a conserved lysine. Surprisingly, biochemical observations implicate an equivalent interaction for the unrelated ATP-dependent activators PAN and PA700.
About this Structure
1YA7 is a Protein complex structure of sequences from Thermoplasma acidophilum and Trypanosoma brucei. Full crystallographic information is available from OCA.
Reference
The 1.9 A structure of a proteasome-11S activator complex and implications for proteasome-PAN/PA700 interactions., Forster A, Masters EI, Whitby FG, Robinson H, Hill CP, Mol Cell. 2005 May 27;18(5):589-99. PMID:15916965
Page seeded by OCA on Thu Mar 20 15:20:15 2008
