1yaa
From Proteopedia
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| - | [[Image:1yaa.gif|left|200px]] | + | [[Image:1yaa.gif|left|200px]] |
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| - | '''ASPARTATE AMINOTRANSFERASE FROM SACCHAROMYCES CEREVISIAE CYTOPLASM''' | + | {{Structure |
| + | |PDB= 1yaa |SIZE=350|CAPTION= <scene name='initialview01'>1yaa</scene>, resolution 2.05Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MAE:MALEIC+ACID'>MAE</scene> and <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Aspartate_transaminase Aspartate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.1 2.6.1.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''ASPARTATE AMINOTRANSFERASE FROM SACCHAROMYCES CEREVISIAE CYTOPLASM''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YAA is a [ | + | 1YAA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YAA OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of Saccharomyces cerevisiae cytosolic aspartate aminotransferase., Jeffery CJ, Barry T, Doonan S, Petsko GA, Ringe D, Protein Sci. 1998 Jun;7(6):1380-7. PMID:[http:// | + | Crystal structure of Saccharomyces cerevisiae cytosolic aspartate aminotransferase., Jeffery CJ, Barry T, Doonan S, Petsko GA, Ringe D, Protein Sci. 1998 Jun;7(6):1380-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9655342 9655342] |
[[Category: Aspartate transaminase]] | [[Category: Aspartate transaminase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:20:17 2008'' |
Revision as of 13:20, 20 March 2008
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| , resolution 2.05Å | |||||||
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| Ligands: | and | ||||||
| Activity: | Aspartate transaminase, with EC number 2.6.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ASPARTATE AMINOTRANSFERASE FROM SACCHAROMYCES CEREVISIAE CYTOPLASM
Overview
The crystal structure of Saccharomyces cerevisiae cytoplasmic aspartate aminotransferase (EC 2.6.1.1) has been determined to 2.05 A resolution in the presence of the cofactor pyridoxal-5'-phosphate and the competitive inhibitor maleate. The structure was solved by the method of molecular replacement. The final value of the crystallographic R-factor after refinement was 23.1% with good geometry of the final model. The yeast cytoplasmic enzyme is a homodimer with two identical active sites containing residues from each subunit. It is found in the "closed" conformation with a bound maleate inhibitor in each active site. It shares the same three-dimensional fold and active site residues as the aspartate aminotransferases from Escherichia coli, chicken cytoplasm, and chicken mitochondria, although it shares less than 50% sequence identity with any of them. The availability of four similar enzyme structures from distant regions of the evolutionary tree provides a measure of tolerated changes that can arise during millions of years of evolution.
About this Structure
1YAA is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of Saccharomyces cerevisiae cytosolic aspartate aminotransferase., Jeffery CJ, Barry T, Doonan S, Petsko GA, Ringe D, Protein Sci. 1998 Jun;7(6):1380-7. PMID:9655342
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