1ybk
From Proteopedia
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| - | [[Image:1ybk.gif|left|200px]] | + | [[Image:1ybk.gif|left|200px]] |
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| - | '''RHCC cocrystallized with CAPB''' | + | {{Structure |
| + | |PDB= 1ybk |SIZE=350|CAPTION= <scene name='initialview01'>1ybk</scene>, resolution 1.45Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=BEQ:N-(CARBOXYMETHYL)-N,N-DIMETHYL-3-[(1-OXODODECYL)AMINO]-1-PROPANAMINIUM INNER SALT'>BEQ</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''RHCC cocrystallized with CAPB''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YBK is a [ | + | 1YBK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylothermus_marinus Staphylothermus marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YBK OCA]. |
==Reference== | ==Reference== | ||
| - | Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)., Ozbek S, Muller JF, Figgemeier E, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:[http:// | + | Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)., Ozbek S, Muller JF, Figgemeier E, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15805603 15805603] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylothermus marinus]] | [[Category: Staphylothermus marinus]] | ||
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[[Category: right hand coiled coil]] | [[Category: right hand coiled coil]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:20:46 2008'' |
Revision as of 13:20, 20 March 2008
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| , resolution 1.45Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RHCC cocrystallized with CAPB
Overview
Crystals of excellent quality are a prerequisite for high-resolution X-ray data. However, in refinement protocols of crystallization conditions it is often difficult to obtain the right combination of, for example, protein concentration, drop size, temperature and additives. A novel approach for optimizing crystal contacts in a most favourable fashion by performing crystallization setups with the zwitterionic surfactant cocoamidoproylbetaine (CAPB) is introduced. In the presence of this surfactant, highly diffracting crystals were obtained. Here, data from a right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution are presented. The addition of CAPB using otherwise identical crystallization conditions and the same X-ray source caused an improvement in resolution from 2.9 to 1.4 A.
About this Structure
1YBK is a Single protein structure of sequence from Staphylothermus marinus. Full crystallographic information is available from OCA.
Reference
Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)., Ozbek S, Muller JF, Figgemeier E, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:15805603
Page seeded by OCA on Thu Mar 20 15:20:46 2008
