1yel
From Proteopedia
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| - | [[Image:1yel.gif|left|200px]] | + | [[Image:1yel.gif|left|200px]] |
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| - | '''Structure of the hypothetical Arabidopsis thaliana protein At1g16640.1''' | + | {{Structure |
| + | |PDB= 1yel |SIZE=350|CAPTION= <scene name='initialview01'>1yel</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structure of the hypothetical Arabidopsis thaliana protein At1g16640.1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YEL is a [ | + | 1YEL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YEL OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the B3 domain from Arabidopsis thaliana protein At1g16640., Waltner JK, Peterson FC, Lytle BL, Volkman BF, Protein Sci. 2005 Sep;14(9):2478-83. Epub 2005 Aug 4. PMID:[http:// | + | Structure of the B3 domain from Arabidopsis thaliana protein At1g16640., Waltner JK, Peterson FC, Lytle BL, Volkman BF, Protein Sci. 2005 Sep;14(9):2478-83. Epub 2005 Aug 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16081658 16081658] |
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Volkman, B F.]] | [[Category: Volkman, B F.]] | ||
[[Category: Waltner, J K.]] | [[Category: Waltner, J K.]] | ||
| - | [[Category: center for eukaryotic structural | + | [[Category: center for eukaryotic structural genomic]] |
[[Category: cesg]] | [[Category: cesg]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
[[Category: unknown function]] | [[Category: unknown function]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:21:50 2008'' |
Revision as of 13:21, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the hypothetical Arabidopsis thaliana protein At1g16640.1
Overview
A novel DNA binding motif, the B3 domain, has been identified in a number of transcription factors specific to higher plant species, and was recently found to define a new protein fold. Here we report the second structure of a B3 domain, that of the Arabidopsis thaliana protein, At1g16640. As part of an effort to 'rescue' structural genomics targets deemed unsuitable for structure determination as full-length proteins, we applied a combined bioinformatic and experimental strategy to identify an optimal construct containing a predicted conserved domain. By screening a series of N- and C-terminally truncated At1g16640 fragments, we isolated a stable folded domain that met our criteria for structural analysis by NMR spectroscopy. The structure of the B3 domain of At1g16640 consists of a seven-stranded beta-sheet arranged in an open barrel and two short alpha-helices, one at each end of the barrel. While At1g16640 is quite distinct from previously characterized B3 domain proteins in terms of amino acid sequence similarity, it adopts the same novel fold that was recently revealed by the RAV1 B3 domain structure. However, putative DNA-binding elements conserved in B3 domains from the RAV, ARF, and ABI3/VP1 subfamilies are largely absent in At1g16640, perhaps suggesting that B3 domains could function in contexts other than transcriptional regulation.
About this Structure
1YEL is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Structure of the B3 domain from Arabidopsis thaliana protein At1g16640., Waltner JK, Peterson FC, Lytle BL, Volkman BF, Protein Sci. 2005 Sep;14(9):2478-83. Epub 2005 Aug 4. PMID:16081658
Page seeded by OCA on Thu Mar 20 15:21:50 2008
