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1ym0
From Proteopedia
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| - | [[Image:1ym0.gif|left|200px]] | + | [[Image:1ym0.gif|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of Earthworm Fibrinolytic Enzyme Component B: a Novel, Glycosylated Two-chained Trypsin''' | + | {{Structure |
| + | |PDB= 1ym0 |SIZE=350|CAPTION= <scene name='initialview01'>1ym0</scene>, resolution 2.06Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Earthworm Fibrinolytic Enzyme Component B: a Novel, Glycosylated Two-chained Trypsin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YM0 is a [ | + | 1YM0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Eisenia_fetida Eisenia fetida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YM0 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of earthworm fibrinolytic enzyme component B: a novel, glycosylated two-chained trypsin., Wang F, Wang C, Li M, Zhang JP, Gui LL, An XM, Chang WR, J Mol Biol. 2005 May 6;348(3):671-85. PMID:[http:// | + | Crystal structure of earthworm fibrinolytic enzyme component B: a novel, glycosylated two-chained trypsin., Wang F, Wang C, Li M, Zhang JP, Gui LL, An XM, Chang WR, J Mol Biol. 2005 May 6;348(3):671-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15826663 15826663] |
[[Category: Eisenia fetida]] | [[Category: Eisenia fetida]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: glycosylation]] | [[Category: glycosylation]] | ||
[[Category: pyroglutamation]] | [[Category: pyroglutamation]] | ||
| - | [[Category: two | + | [[Category: two chain]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:24:37 2008'' |
Revision as of 13:24, 20 March 2008
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| , resolution 2.06Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Earthworm Fibrinolytic Enzyme Component B: a Novel, Glycosylated Two-chained Trypsin
Overview
The earthworm fibrinolytic enzyme (EFE), belonging to a group of serine proteases with strong fibrinolytic activity, has been used in a mixture as an oral drug for prevention and treatment of thrombosis in East Asia. The EFE component b (EFE-b) is one of seven EFE components from Eisenia fetida, and among them it has nearly the highest fibrinolytic activity. Here, we report its crystal structure at a resolution of 2.06A. The structural analysis shows that EFE-b should be classified as a trypsin from earthworm. However, it is distinct from other trypsins. It is a two-chained protease with an N-terminal, pyroglutamated light chain and an N-glycosylated heavy chain. Furthermore, the heavy chain contains a novel structural motif, an eight-membered ring resulting from a disulfide bridge between two neighboring cysteine residues, and a cis peptide bond exists between these two cysteine residues. The crystal structure of EFE-b provides the structural basis for its high level of stability and reveals its complicated post-translational modifications in earthworm. This structure is the first reported for a glycosylated two-chained trypsin, which may provide useful clues to explain the origin and evolution of the chymotrypsin family.
About this Structure
1YM0 is a Protein complex structure of sequences from Eisenia fetida. Full crystallographic information is available from OCA.
Reference
Crystal structure of earthworm fibrinolytic enzyme component B: a novel, glycosylated two-chained trypsin., Wang F, Wang C, Li M, Zhang JP, Gui LL, An XM, Chang WR, J Mol Biol. 2005 May 6;348(3):671-85. PMID:15826663
Page seeded by OCA on Thu Mar 20 15:24:37 2008
Categories: Eisenia fetida | Protein complex | An, X M. | Chang, W R. | Gui, L L. | Li, M. | Wang, C. | Wang, F. | Zhang, J P. | MG | SO4 | Cis peptide bond | Eight-membered ring | Glycosylation | Pyroglutamation | Two chain
