2buo
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Immature HIV particles bud from infected cells after assembly at the, cytoplasmic side of cellular membranes. This assembly is driven by, interactions between Gag polyproteins. Mature particles, each containing a, characteristic conical core, are later generated by proteolytic maturation, of Gag in the virion. The C-terminal domain of the HIV-1 capsid protein, (C-CA) has been shown to contain oligomerization determinants essential, for particle assembly. Here we report the 1.7-A-resolution crystal, structure of C-CA in complex with a peptide capable of inhibiting, immature- and mature-like particle assembly in vitro. The peptide inserts, as an amphipathic alpha-helix into a conserved hydrophobic groove of C-CA, resulting in formation of a compact five-helix bundle with altered dimeric, .. | + | Immature HIV particles bud from infected cells after assembly at the, cytoplasmic side of cellular membranes. This assembly is driven by, interactions between Gag polyproteins. Mature particles, each containing a, characteristic conical core, are later generated by proteolytic maturation, of Gag in the virion. The C-terminal domain of the HIV-1 capsid protein, (C-CA) has been shown to contain oligomerization determinants essential, for particle assembly. Here we report the 1.7-A-resolution crystal, structure of C-CA in complex with a peptide capable of inhibiting, immature- and mature-like particle assembly in vitro. The peptide inserts, as an amphipathic alpha-helix into a conserved hydrophobic groove of C-CA, resulting in formation of a compact five-helix bundle with altered dimeric, interactions. This structure thus reveals the details of an allosteric, site in the HIV capsid protein that can be targeted for antiviral therapy. |
==About this Structure== | ==About this Structure== | ||
| - | 2BUO is a | + | 2BUO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] with ACY as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BUO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: polyprotein]] | [[Category: polyprotein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:12:58 2007'' |
Revision as of 11:07, 5 November 2007
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HIV-1 CAPSID C-TERMINAL DOMAIN IN COMPLEX WITH AN INHIBITOR OF PARTICLE ASSEMBLY
Overview
Immature HIV particles bud from infected cells after assembly at the, cytoplasmic side of cellular membranes. This assembly is driven by, interactions between Gag polyproteins. Mature particles, each containing a, characteristic conical core, are later generated by proteolytic maturation, of Gag in the virion. The C-terminal domain of the HIV-1 capsid protein, (C-CA) has been shown to contain oligomerization determinants essential, for particle assembly. Here we report the 1.7-A-resolution crystal, structure of C-CA in complex with a peptide capable of inhibiting, immature- and mature-like particle assembly in vitro. The peptide inserts, as an amphipathic alpha-helix into a conserved hydrophobic groove of C-CA, resulting in formation of a compact five-helix bundle with altered dimeric, interactions. This structure thus reveals the details of an allosteric, site in the HIV capsid protein that can be targeted for antiviral therapy.
About this Structure
2BUO is a Protein complex structure of sequences from Human immunodeficiency virus 1 with ACY as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
The HIV-1 capsid protein C-terminal domain in complex with a virus assembly inhibitor., Ternois F, Sticht J, Duquerroy S, Krausslich HG, Rey FA, Nat Struct Mol Biol. 2005 Aug;12(8):678-82. Epub 2005 Jul 24. PMID:16041386
Page seeded by OCA on Mon Nov 5 13:12:58 2007
