1yu5
From Proteopedia
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| - | [[Image:1yu5.gif|left|200px]] | + | [[Image:1yu5.gif|left|200px]] |
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| - | '''Crystal Structure of the Headpiece Domain of Chicken Villin''' | + | {{Structure |
| + | |PDB= 1yu5 |SIZE=350|CAPTION= <scene name='initialview01'>1yu5</scene>, resolution 1.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Crystal Structure of the Headpiece Domain of Chicken Villin''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YU5 is a [ | + | 1YU5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YU5 OCA]. |
==Reference== | ==Reference== | ||
| - | High-resolution crystal structures of villin headpiece and mutants with reduced F-actin binding activity., Meng J, Vardar D, Wang Y, Guo HC, Head JF, McKnight CJ, Biochemistry. 2005 Sep 13;44(36):11963-73. PMID:[http:// | + | High-resolution crystal structures of villin headpiece and mutants with reduced F-actin binding activity., Meng J, Vardar D, Wang Y, Guo HC, Head JF, McKnight CJ, Biochemistry. 2005 Sep 13;44(36):11963-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16142894 16142894] |
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: alpha helix]] | [[Category: alpha helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:27:31 2008'' |
Revision as of 13:27, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Headpiece Domain of Chicken Villin
Overview
Villin-type headpiece domains are approximately 70 amino acid modular motifs found at the C terminus of a variety of actin cytoskeleton-associated proteins. The headpiece domain of villin, a protein found in the actin bundles of the brush border epithelium, is of interest both as a compact F-actin binding domain and as a model folded protein. We have determined the high-resolution crystal structures of chicken villin headpiece (HP67) at 1.4 A resolution as well as two mutants, R37A and W64Y, at 1.45 and 1.5 A resolution, respectively. Replacement of R37 causes a 5-fold reduction in F-actin binding affinity in sedimentation assays. Replacement of W64 results in a much more drastic reduction in F-actin binding affinity without significant changes in headpiece structure or stability. The detailed comparison of these crystal structures with each other and to our previously determined NMR structures of HP67 and the 35-residue autonomously folding subdomain in villin headpiece, HP35, provides the details of the headpiece fold and further defines the F-actin binding site of villin-type headpiece domains.
About this Structure
1YU5 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
High-resolution crystal structures of villin headpiece and mutants with reduced F-actin binding activity., Meng J, Vardar D, Wang Y, Guo HC, Head JF, McKnight CJ, Biochemistry. 2005 Sep 13;44(36):11963-73. PMID:16142894
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