1yuh

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[[Image:1yuh.jpg|left|200px]]<br /><applet load="1yuh" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1yuh.jpg|left|200px]]
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caption="1yuh, resolution 3.0&Aring;" />
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'''FAB FRAGMENT'''<br />
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{{Structure
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|PDB= 1yuh |SIZE=350|CAPTION= <scene name='initialview01'>1yuh</scene>, resolution 3.0&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NP:4-HYDROXY-3-NITROPHENYLACETYL-EPSILON-AMINOCAPROIC ACID'>NP</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''FAB FRAGMENT'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1YUH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NP:'>NP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YUH OCA].
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1YUH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YUH OCA].
==Reference==
==Reference==
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Structural analysis of affinity maturation: the three-dimensional structures of complexes of an anti-nitrophenol antibody., Yuhasz SC, Parry C, Strand M, Amzel LM, Mol Immunol. 1995 Oct;32(14-15):1143-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8544863 8544863]
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Structural analysis of affinity maturation: the three-dimensional structures of complexes of an anti-nitrophenol antibody., Yuhasz SC, Parry C, Strand M, Amzel LM, Mol Immunol. 1995 Oct;32(14-15):1143-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8544863 8544863]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Amzel, L M.]]
[[Category: Amzel, L M.]]
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[[Category: lambda light chain]]
[[Category: lambda light chain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:09:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:27:39 2008''

Revision as of 13:27, 20 March 2008


PDB ID 1yuh

Drag the structure with the mouse to rotate
, resolution 3.0Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



FAB FRAGMENT


Overview

Affinity maturation of the immune response to nitrophenol-containing antigens has been extensively investigated. Significant strides made during the past several years with the advent of PCR technology have provided a wealth of biochemical knowledge. Structural investigations of the phenomena have however been limited. We have determined the three-dimensional structure of the Fab fragment of 88C6/12, an anti-4-hydroxy-3-nitrophenyl acetic acid antibody complexed with the immunizing hapten and with a heteroclitic iodinated hapten. The crystallographic structure of the complexes reveals that the binding is stabilized by a number of hydrogen bonds and extensive van der Waals interactions between the hapten and the antibody. In addition, the Fab binding pocket contains a region of positive electrostatic potential well suited for interaction with the predominant resonance form of the nitrophenyl ring system. The observed heteroclicity towards the iodinated hapten is not a direct result of iodine-protein interactions, but results from the enhanced stability in the iodinated ring of the resonance form that binds the antibody. In addition this investigation provides a rationale for the strong preference for the substitution in the heavy chain from the germ-line gene encoded Trp 33 to Leu 33 in the mature anti-nitrophenol response.

About this Structure

1YUH is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structural analysis of affinity maturation: the three-dimensional structures of complexes of an anti-nitrophenol antibody., Yuhasz SC, Parry C, Strand M, Amzel LM, Mol Immunol. 1995 Oct;32(14-15):1143-55. PMID:8544863

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