1yvg
From Proteopedia
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| - | [[Image:1yvg.gif|left|200px]] | + | [[Image:1yvg.gif|left|200px]] |
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| - | '''Structural analysis of the catalytic domain of tetanus neurotoxin''' | + | {{Structure |
| + | |PDB= 1yvg |SIZE=350|CAPTION= <scene name='initialview01'>1yvg</scene>, resolution 2.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] | ||
| + | |GENE= tetX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1513 Clostridium tetani]) | ||
| + | }} | ||
| + | |||
| + | '''Structural analysis of the catalytic domain of tetanus neurotoxin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YVG is a [ | + | 1YVG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YVG OCA]. |
==Reference== | ==Reference== | ||
| - | Structural analysis of the catalytic domain of tetanus neurotoxin., Rao KN, Kumaran D, Binz T, Swaminathan S, Toxicon. 2005 Jun 1;45(7):929-39. Epub 2005 Apr 13. PMID:[http:// | + | Structural analysis of the catalytic domain of tetanus neurotoxin., Rao KN, Kumaran D, Binz T, Swaminathan S, Toxicon. 2005 Jun 1;45(7):929-39. Epub 2005 Apr 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15904688 15904688] |
[[Category: Clostridium tetani]] | [[Category: Clostridium tetani]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: clostridium neurotoxins; tetanus neurotoxin; zinc metalloprotease; dual-wavelength anomalous dispersion (dad)]] | [[Category: clostridium neurotoxins; tetanus neurotoxin; zinc metalloprotease; dual-wavelength anomalous dispersion (dad)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:28:00 2008'' |
Revision as of 13:28, 20 March 2008
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| , resolution 2.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | tetX (Clostridium tetani) | ||||||
| Activity: | Tentoxilysin, with EC number 3.4.24.68 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural analysis of the catalytic domain of tetanus neurotoxin
Overview
Clostridium neurotoxins, comprising the tetanus neurotoxin and the seven antigenically distinct botulinum neurotoxins (BoNT/A-G), are among the known most potent bacterial protein toxins to humans. Although they have similar function, sequences and three-dimensional structures, the substrate specificity and the selectivity of peptide bond cleavage are different and unique. Tetanus and botulinum type B neurotoxins enzymatically cleave the same substrate, vesicle-associated membrane protein, at the same peptide bond though the optimum length of substrate peptide required for cleavage by them is different. Here, we present the first experimentally determined three-dimensional structure of the catalytic domain of tetanus neurotoxin and analyze its active site. The structure provides insight into the active site of tetanus toxin's proteolytic activity and the importance of the nucleophilic water and the role of the zinc ion. The probable reason for different modes of binding of vesicle-associated membrane protein to botulinum neurotoxin type B and the tetanus toxin is discussed. The structure provides a basis for designing a novel recombinant vaccine or structure-based drugs for tetanus.
About this Structure
1YVG is a Single protein structure of sequence from Clostridium tetani. Full crystallographic information is available from OCA.
Reference
Structural analysis of the catalytic domain of tetanus neurotoxin., Rao KN, Kumaran D, Binz T, Swaminathan S, Toxicon. 2005 Jun 1;45(7):929-39. Epub 2005 Apr 13. PMID:15904688
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