1yvu

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[[Image:1yvu.gif|left|200px]]<br /><applet load="1yvu" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1yvu.gif|left|200px]]
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caption="1yvu, resolution 2.90&Aring;" />
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'''Crystal structure of A. aeolicus Argonaute'''<br />
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{{Structure
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|PDB= 1yvu |SIZE=350|CAPTION= <scene name='initialview01'>1yvu</scene>, resolution 2.90&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal structure of A. aeolicus Argonaute'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1YVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus_vf5 Aquifex aeolicus vf5] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YVU OCA].
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1YVU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus_vf5 Aquifex aeolicus vf5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YVU OCA].
==Reference==
==Reference==
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Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage., Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, Dauter Z, Tuschl T, Patel DJ, Mol Cell. 2005 Aug 5;19(3):405-19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16061186 16061186]
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Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage., Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, Dauter Z, Tuschl T, Patel DJ, Mol Cell. 2005 Aug 5;19(3):405-19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16061186 16061186]
[[Category: Aquifex aeolicus vf5]]
[[Category: Aquifex aeolicus vf5]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: rnase h fold]]
[[Category: rnase h fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:09:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:28:08 2008''

Revision as of 13:28, 20 March 2008

Image:1yvu.gif


PDB ID 1yvu

Drag the structure with the mouse to rotate
, resolution 2.90Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Crystal structure of A. aeolicus Argonaute


Overview

Argonaute (Ago) proteins constitute a key component of the RNA-induced silencing complex (RISC). We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and cleavage studies, which establish this eubacterial Ago as a bona fide guide DNA strand-mediated site-specific RNA endonuclease. We have generated a stereochemically robust model of the complex, where the guide DNA-mRNA duplex is positioned within a basic channel spanning the bilobal interface, such that the 5' phosphate of the guide strand can be anchored in a basic pocket, and the mRNA can be positioned for site-specific cleavage by RNase H-type divalent cation-coordinated catalytic Asp residues of the PIWI domain. Domain swap experiments involving chimeras of human Ago (hAgo1) and cleavage-competent hAgo2 reinforce the role of the PIWI domain in "slicer" activity. We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC.

About this Structure

1YVU is a Single protein structure of sequence from Aquifex aeolicus vf5. Full crystallographic information is available from OCA.

Reference

Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage., Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, Dauter Z, Tuschl T, Patel DJ, Mol Cell. 2005 Aug 5;19(3):405-19. PMID:16061186

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