1uu4
From Proteopedia
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==Overview== | ==Overview== | ||
| - | As part of an ongoing enzyme discovery program to investigate the, properties and catalytic mechanism of glycoside hydrolase family 12 (GH, 12) endoglucanases, a GH family that contains several cellulases that are, of interest in industrial applications, we have solved four new crystal, structures of wild-type Humicola grisea Cel12A in complexes formed by, soaking with cellobiose, cellotetraose, cellopentaose, and a thio-linked, cellotetraose derivative (G2SG2). These complex structures allow mapping, of the non-covalent interactions between the enzyme and the glucosyl chain, bound in subsites -4 to +2 of the enzyme, and shed light on the mechanism, and function of GH 12 cellulases. The unhydrolysed cellopentaose and the, G2SG2 cello-oligomers span the active site of the catalytically ... | + | As part of an ongoing enzyme discovery program to investigate the, properties and catalytic mechanism of glycoside hydrolase family 12 (GH, 12) endoglucanases, a GH family that contains several cellulases that are, of interest in industrial applications, we have solved four new crystal, structures of wild-type Humicola grisea Cel12A in complexes formed by, soaking with cellobiose, cellotetraose, cellopentaose, and a thio-linked, cellotetraose derivative (G2SG2). These complex structures allow mapping, of the non-covalent interactions between the enzyme and the glucosyl chain, bound in subsites -4 to +2 of the enzyme, and shed light on the mechanism, and function of GH 12 cellulases. The unhydrolysed cellopentaose and the, G2SG2 cello-oligomers span the active site of the catalytically active, H.grisea Cel12A enzyme, with the pyranoside bound in subsite -1 displaying, a S31 skew boat conformation. After soaking in cellotetraose, the, cello-oligomer that is found bound in site -4 to -1 contains a, beta-1,3-linkage between the two cellobiose units in the oligomer, which, is believed to have been formed by a transglycosylation reaction that has, occurred during the ligand soak of the protein crystals. The close fit of, this ligand and the binding sites occupied suggest a novel mixed, beta-glucanase activity for this enzyme. |
==About this Structure== | ==About this Structure== | ||
| - | 1UU4 is a | + | 1UU4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Humicola_grisea Humicola grisea] with PG4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Structure known Active Site: 1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UU4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ligand complex]] | [[Category: ligand complex]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:13:28 2007'' |
Revision as of 11:08, 5 November 2007
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X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF HUMICOLA GRISEA CEL12A IN COMPLEX WITH CELLOBIOSE
Overview
As part of an ongoing enzyme discovery program to investigate the, properties and catalytic mechanism of glycoside hydrolase family 12 (GH, 12) endoglucanases, a GH family that contains several cellulases that are, of interest in industrial applications, we have solved four new crystal, structures of wild-type Humicola grisea Cel12A in complexes formed by, soaking with cellobiose, cellotetraose, cellopentaose, and a thio-linked, cellotetraose derivative (G2SG2). These complex structures allow mapping, of the non-covalent interactions between the enzyme and the glucosyl chain, bound in subsites -4 to +2 of the enzyme, and shed light on the mechanism, and function of GH 12 cellulases. The unhydrolysed cellopentaose and the, G2SG2 cello-oligomers span the active site of the catalytically active, H.grisea Cel12A enzyme, with the pyranoside bound in subsite -1 displaying, a S31 skew boat conformation. After soaking in cellotetraose, the, cello-oligomer that is found bound in site -4 to -1 contains a, beta-1,3-linkage between the two cellobiose units in the oligomer, which, is believed to have been formed by a transglycosylation reaction that has, occurred during the ligand soak of the protein crystals. The close fit of, this ligand and the binding sites occupied suggest a novel mixed, beta-glucanase activity for this enzyme.
About this Structure
1UU4 is a Single protein structure of sequence from Humicola grisea with PG4 as ligand. Active as Cellulase, with EC number 3.2.1.4 Structure known Active Site: 1. Full crystallographic information is available from OCA.
Reference
Crystal complex structures reveal how substrate is bound in the -4 to the +2 binding sites of Humicola grisea Cel12A., Sandgren M, Berglund GI, Shaw A, Stahlberg J, Kenne L, Desmet T, Mitchinson C, J Mol Biol. 2004 Oct 1;342(5):1505-17. PMID:15364577
Page seeded by OCA on Mon Nov 5 13:13:28 2007
