1yyf
From Proteopedia
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| - | [[Image:1yyf.gif|left|200px]] | + | [[Image:1yyf.gif|left|200px]] |
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| - | '''Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects''' | + | {{Structure |
| + | |PDB= 1yyf |SIZE=350|CAPTION= <scene name='initialview01'>1yyf</scene>, resolution 4.16Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= hslU, htpI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), hslV, clpQ, codW ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | }} | ||
| + | |||
| + | '''Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YYF is a [ | + | 1YYF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1YYF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YYF OCA]. |
==Reference== | ==Reference== | ||
| - | Correction of X-ray intensities from an HslV-HslU co-crystal containing lattice-translocation defects., Wang J, Rho SH, Park HH, Eom SH, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):932-41. Epub 2005, Jun 24. PMID:[http:// | + | Correction of X-ray intensities from an HslV-HslU co-crystal containing lattice-translocation defects., Wang J, Rho SH, Park HH, Eom SH, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):932-41. Epub 2005, Jun 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15983416 15983416] |
[[Category: AAA+ Proteases]] | [[Category: AAA+ Proteases]] | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
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[[Category: quaternary structure]] | [[Category: quaternary structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:29:03 2008'' |
Revision as of 13:29, 20 March 2008
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| , resolution 4.16Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | hslU, htpI (Escherichia coli), hslV, clpQ, codW (Bacillus subtilis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects
Overview
Because of lattice-translocation defects, two identical but translated lattices can coexist as a single coherent mosaic block in a crystal. The observed structure in such cases is a weighted sum of two identical but translated structures, one from each lattice; the observed structure factors are a weighted vector sum of the structure factors with identical unit amplitudes but shifted phases. The correction of X-ray intensities from a single crystal containing these defects of the hybrid HslV-HslU complex, which consists of Escherichia coli HslU and Bacillus subtilis HslV (also known as CodW), is reported. When intensities are not corrected, a biologically irrelevant complex (with CodW from one lattice and HslU from another) is implied to exist. Only upon correction does a biologically functional CodW-HslU complex structure emerge.
About this Structure
1YYF is a Protein complex structure of sequences from Bacillus subtilis and Escherichia coli. The following page contains interesting information on the relation of 1YYF with [AAA+ Proteases]. Full crystallographic information is available from OCA.
Reference
Correction of X-ray intensities from an HslV-HslU co-crystal containing lattice-translocation defects., Wang J, Rho SH, Park HH, Eom SH, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):932-41. Epub 2005, Jun 24. PMID:15983416
Page seeded by OCA on Thu Mar 20 15:29:03 2008
