1yz9
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1yz9.gif|left|200px]] | + | [[Image:1yz9.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of RNase III mutant E110Q from Aquifex aeolicus complexed with double stranded RNA at 2.1-Angstrom Resolution''' | + | {{Structure |
| + | |PDB= 1yz9 |SIZE=350|CAPTION= <scene name='initialview01'>1yz9</scene>, resolution 2.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] | ||
| + | |GENE= rnc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of RNase III mutant E110Q from Aquifex aeolicus complexed with double stranded RNA at 2.1-Angstrom Resolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1YZ9 is a [ | + | 1YZ9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YZ9 OCA]. |
==Reference== | ==Reference== | ||
| - | Intermediate states of ribonuclease III in complex with double-stranded RNA., Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X, Structure. 2005 Oct;13(10):1435-42. PMID:[http:// | + | Intermediate states of ribonuclease III in complex with double-stranded RNA., Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X, Structure. 2005 Oct;13(10):1435-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16216575 16216575] |
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
[[Category: Ribonuclease III]] | [[Category: Ribonuclease III]] | ||
| Line 26: | Line 35: | ||
[[Category: rna interference]] | [[Category: rna interference]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:29:21 2008'' |
Revision as of 13:29, 20 March 2008
| |||||||
| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | rnc (Aquifex aeolicus) | ||||||
| Activity: | Ribonuclease III, with EC number 3.1.26.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of RNase III mutant E110Q from Aquifex aeolicus complexed with double stranded RNA at 2.1-Angstrom Resolution
Overview
Bacterial ribonuclease III (RNase III) can affect RNA structure and gene expression in either of two ways: as a processing enzyme that cleaves double-stranded (ds) RNA, or as a binding protein that binds but does not cleave dsRNA. We previously proposed a model of the catalytic complex of RNase III with dsRNA based on three crystal structures, including the endonuclease domain of RNase III with and without bound metal ions and a dsRNA binding protein complexed with dsRNA. We also reported a noncatalytic assembly observed in the crystal structure of an RNase III mutant, which binds but does not cleave dsRNA, complexed with dsRNA. We hypothesize that the RNase III*dsRNA complex can exist in two functional forms, a catalytic complex and a noncatalytic assembly, and that in between the two forms there may be intermediate states. Here, we present four crystal structures of RNase III complexed with dsRNA, representing possible intermediates.
About this Structure
1YZ9 is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Intermediate states of ribonuclease III in complex with double-stranded RNA., Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X, Structure. 2005 Oct;13(10):1435-42. PMID:16216575
Page seeded by OCA on Thu Mar 20 15:29:21 2008
