1z01
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1z01.gif|left|200px]] | + | [[Image:1z01.gif|left|200px]] |
| - | + | ||
| - | '''2-Oxoquinoline 8-Monooxygenase Component: Active site Modulation by Rieske-[2fe-2S] Center Oxidation/Reduction''' | + | {{Structure |
| + | |PDB= 1z01 |SIZE=350|CAPTION= <scene name='initialview01'>1z01</scene>, resolution 1.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene> and <scene name='pdbligand=FES:FE2/S2 (INORGANIC) CLUSTER'>FES</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/2-hydroxyquinoline_8-monooxygenase 2-hydroxyquinoline 8-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.61 1.14.13.61] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''2-Oxoquinoline 8-Monooxygenase Component: Active site Modulation by Rieske-[2fe-2S] Center Oxidation/Reduction''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1Z01 is a [ | + | 1Z01 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z01 OCA]. |
==Reference== | ==Reference== | ||
| - | 2-Oxoquinoline 8-monooxygenase oxygenase component: active site modulation by Rieske-[2Fe-2S] center oxidation/reduction., Martins BM, Svetlitchnaia T, Dobbek H, Structure. 2005 May;13(5):817-24. PMID:[http:// | + | 2-Oxoquinoline 8-monooxygenase oxygenase component: active site modulation by Rieske-[2Fe-2S] center oxidation/reduction., Martins BM, Svetlitchnaia T, Dobbek H, Structure. 2005 May;13(5):817-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15893671 15893671] |
[[Category: 2-hydroxyquinoline 8-monooxygenase]] | [[Category: 2-hydroxyquinoline 8-monooxygenase]] | ||
[[Category: Pseudomonas putida]] | [[Category: Pseudomonas putida]] | ||
| Line 24: | Line 33: | ||
[[Category: substrate bound complex]] | [[Category: substrate bound complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:29:37 2008'' |
Revision as of 13:29, 20 March 2008
| |||||||
| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | 2-hydroxyquinoline 8-monooxygenase, with EC number 1.14.13.61 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
2-Oxoquinoline 8-Monooxygenase Component: Active site Modulation by Rieske-[2fe-2S] Center Oxidation/Reduction
Overview
2-Oxoquinoline 8-monooxygenase is a Rieske non-heme iron oxygenase that catalyzes the NADH-dependent oxidation of the N-heterocyclic aromatic compound 2-oxoquinoline to 8-hydroxy-2-oxoquinoline in the soil bacterium Pseudomonas putida 86. The crystal structure of the oxygenase component of 2-oxoquinoline 8-monooxygenase shows a ring-shaped, C3-symmetric arrangement in which the mononuclear Fe(II) ion active site of one monomer is at a distance of 13 A from the Rieske-[2Fe-2S] center of a second monomer. Structural analyses of oxidized, reduced, and substrate bound states reveal the molecular bases for a new function of Fe-S clusters. Reduction of the Rieske center modulates the mononuclear Fe through a chain of conformational changes across the subunit interface, resulting in the displacement of Fe and its histidine ligand away from the substrate binding site. This creates an additional coordination site at the mononuclear Fe(II) ion and can open a pathway for dioxygen to bind in the substrate-containing active site.
About this Structure
1Z01 is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.
Reference
2-Oxoquinoline 8-monooxygenase oxygenase component: active site modulation by Rieske-[2Fe-2S] center oxidation/reduction., Martins BM, Svetlitchnaia T, Dobbek H, Structure. 2005 May;13(5):817-24. PMID:15893671
Page seeded by OCA on Thu Mar 20 15:29:37 2008
