1z1a
From Proteopedia
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| - | [[Image:1z1a.gif|left|200px]] | + | [[Image:1z1a.gif|left|200px]] |
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| - | '''S. cerevisiae Sir1 ORC-interaction domain''' | + | {{Structure |
| + | |PDB= 1z1a |SIZE=350|CAPTION= <scene name='initialview01'>1z1a</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SIR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''S. cerevisiae Sir1 ORC-interaction domain''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Z1A is a [ | + | 1Z1A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1A OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of the Sir1-origin recognition complex interaction in transcriptional silencing., Hou Z, Bernstein DA, Fox CA, Keck JL, Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8489-94. Epub 2005 Jun 2. PMID:[http:// | + | Structural basis of the Sir1-origin recognition complex interaction in transcriptional silencing., Hou Z, Bernstein DA, Fox CA, Keck JL, Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8489-94. Epub 2005 Jun 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15932939 15932939] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: novel fold]] | [[Category: novel fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:30:05 2008'' |
Revision as of 13:30, 20 March 2008
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| , resolution 2.50Å | |||||||
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| Gene: | SIR1 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
S. cerevisiae Sir1 ORC-interaction domain
Overview
The Sir1 protein plays a key role in establishing a silent chromatin structure at the cryptic mating-type loci HMR and HML in Saccharomyces cerevisiae by interacting with the bromo-adjacent homology (BAH) domain of the Orc1p subunit of the origin recognition complex (ORC). Here, we present the high-resolution crystal structures of the ORC interaction region (OIR) of Sir1p and that of the complex formed between the OIR and BAH domains. Amino acids within the OIR previously shown to be required for a Sir1p/ORC interaction are presented on a conserved, convex surface that forms a complementary interface with a concave region of the Orc1 BAH domain that is critical for transcriptional silencing. The OIR/BAH interaction surface comprises a network of hydrophobic and polar/ionic interactions between discrete structural modules in each protein and involves several residues that were not implicated in previous studies. These data provide important structural insights into a protein-protein interaction critical for the formation of a specialized chromatin domain within eukaryotic chromosomes.
About this Structure
1Z1A is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural basis of the Sir1-origin recognition complex interaction in transcriptional silencing., Hou Z, Bernstein DA, Fox CA, Keck JL, Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8489-94. Epub 2005 Jun 2. PMID:15932939
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