1z25

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[[Image:1z25.gif|left|200px]]<br /><applet load="1z25" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1z25.gif|left|200px]]
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caption="1z25, resolution 2.7&Aring;" />
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'''Structure of P.furiosus Argonaute with bound Mn2+'''<br />
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{{Structure
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|PDB= 1z25 |SIZE=350|CAPTION= <scene name='initialview01'>1z25</scene>, resolution 2.7&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Structure of P.furiosus Argonaute with bound Mn2+'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1Z25 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z25 OCA].
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1Z25 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z25 OCA].
==Reference==
==Reference==
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Purified Argonaute2 and an siRNA form recombinant human RISC., Rivas FV, Tolia NH, Song JJ, Aragon JP, Liu J, Hannon GJ, Joshua-Tor L, Nat Struct Mol Biol. 2005 Apr;12(4):340-9. Epub 2005 Mar 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15800637 15800637]
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Purified Argonaute2 and an siRNA form recombinant human RISC., Rivas FV, Tolia NH, Song JJ, Aragon JP, Liu J, Hannon GJ, Joshua-Tor L, Nat Struct Mol Biol. 2005 Apr;12(4):340-9. Epub 2005 Mar 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15800637 15800637]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: rnaseh]]
[[Category: rnaseh]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:11:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:30:19 2008''

Revision as of 13:30, 20 March 2008

Image:1z25.gif


PDB ID 1z25

Drag the structure with the mouse to rotate
, resolution 2.7Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Structure of P.furiosus Argonaute with bound Mn2+


Overview

Genetic, biochemical and structural studies have implicated Argonaute proteins as the catalytic core of the RNAi effector complex, RISC. Here we show that recombinant, human Argonaute2 can combine with a small interfering RNA (siRNA) to form minimal RISC that accurately cleaves substrate RNAs. Recombinant RISC shows many of the properties of RISC purified from human or Drosophila melanogaster cells but also has surprising features. It shows no stimulation by ATP, suggesting that factors promoting product release are missing from the recombinant enzyme. The active site is made up of a unique Asp-Asp-His (DDH) motif. In the RISC reconstitution system, the siRNA 5' phosphate is important for the stability and the fidelity of the complex but is not essential for the creation of an active enzyme. These studies demonstrate that Argonaute proteins catalyze mRNA cleavage within RISC and provide a source of recombinant enzyme for detailed biochemical studies of the RNAi effector complex.

About this Structure

1Z25 is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.

Reference

Purified Argonaute2 and an siRNA form recombinant human RISC., Rivas FV, Tolia NH, Song JJ, Aragon JP, Liu J, Hannon GJ, Joshua-Tor L, Nat Struct Mol Biol. 2005 Apr;12(4):340-9. Epub 2005 Mar 30. PMID:15800637

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