1z3h
From Proteopedia
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| - | [[Image:1z3h.gif|left|200px]] | + | [[Image:1z3h.gif|left|200px]] |
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| - | '''The exportin Cse1 in its cargo-free, cytoplasmic state''' | + | {{Structure |
| + | |PDB= 1z3h |SIZE=350|CAPTION= <scene name='initialview01'>1z3h</scene>, resolution 3.100Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= Cse1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''The exportin Cse1 in its cargo-free, cytoplasmic state''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Z3H is a [ | + | 1Z3H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3H OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of the nuclear export receptor Cse1 in its cytosolic state reveals a closed conformation incompatible with cargo binding., Cook A, Fernandez E, Lindner D, Ebert J, Schlenstedt G, Conti E, Mol Cell. 2005 Apr 29;18(3):355-67. PMID:[http:// | + | The structure of the nuclear export receptor Cse1 in its cytosolic state reveals a closed conformation incompatible with cargo binding., Cook A, Fernandez E, Lindner D, Ebert J, Schlenstedt G, Conti E, Mol Cell. 2005 Apr 29;18(3):355-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15866177 15866177] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: nuclear transport]] | [[Category: nuclear transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:30:46 2008'' |
Revision as of 13:30, 20 March 2008
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| , resolution 3.100Å | |||||||
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| Ligands: | |||||||
| Gene: | Cse1 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The exportin Cse1 in its cargo-free, cytoplasmic state
Overview
Cse1 mediates nuclear export of importin alpha, the nuclear localization signal (NLS) import adaptor. We report the 3.1 A resolution structure of cargo-free Cse1, representing this HEAT repeat protein in its cytosolic state. Cse1 is compact, consisting of N- and C-terminal arches that interact to form a ring. Comparison with the structure of cargo-bound Cse1 shows a major conformational change leading to opening of the structure upon cargo binding. The largest structural changes occur within a hinge region centered at HEAT repeat 8. This repeat contains a conserved insertion that connects the RanGTP and importin alpha contact sites and that is essential for binding. In the cargo-free state, the RanGTP binding sites are occluded and the importin alpha sites are distorted. Mutations that destabilize the N- to C-terminal interaction uncouple importin alpha and Ran binding, suggesting that the closed conformation prevents association with importin alpha.
About this Structure
1Z3H is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The structure of the nuclear export receptor Cse1 in its cytosolic state reveals a closed conformation incompatible with cargo binding., Cook A, Fernandez E, Lindner D, Ebert J, Schlenstedt G, Conti E, Mol Cell. 2005 Apr 29;18(3):355-67. PMID:15866177
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