1z5w

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[[Image:1z5w.gif|left|200px]]<br /><applet load="1z5w" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1z5w.gif|left|200px]]
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caption="1z5w, resolution 3.00&Aring;" />
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'''Crystal Structure of gamma-tubulin bound to GTP'''<br />
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{{Structure
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|PDB= 1z5w |SIZE=350|CAPTION= <scene name='initialview01'>1z5w</scene>, resolution 3.00&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene>
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|ACTIVITY=
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|GENE= TUBG1, TUBG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Crystal Structure of gamma-tubulin bound to GTP'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1Z5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GTP:'>GTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z5W OCA].
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1Z5W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z5W OCA].
==Reference==
==Reference==
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Insights into microtubule nucleation from the crystal structure of human gamma-tubulin., Aldaz H, Rice LM, Stearns T, Agard DA, Nature. 2005 May 26;435(7041):523-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15917813 15917813]
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Insights into microtubule nucleation from the crystal structure of human gamma-tubulin., Aldaz H, Rice LM, Stearns T, Agard DA, Nature. 2005 May 26;435(7041):523-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15917813 15917813]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: complex with gtp]]
[[Category: complex with gtp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:12:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:31:32 2008''

Revision as of 13:31, 20 March 2008

Image:1z5w.gif


PDB ID 1z5w

Drag the structure with the mouse to rotate
, resolution 3.00Å
Ligands: and
Gene: TUBG1, TUBG (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of gamma-tubulin bound to GTP


Overview

Microtubules are hollow polymers of alphabeta-tubulin that show GTP-dependent assembly dynamics and comprise a critical part of the eukaryotic cytoskeleton. Initiation of new microtubules in vivo requires gamma-tubulin, organized as an oligomer within the 2.2-MDa gamma-tubulin ring complex (gamma-TuRC) of higher eukaryotes. Structural insight is lacking regarding gamma-tubulin, its oligomerization and how it promotes microtubule assembly. Here we report the 2.7-A crystal structure of human gamma-tubulin bound to GTP-gammaS (a non-hydrolysable GTP analogue). We observe a 'curved' conformation for gamma-tubulin-GTPgammaS, similar to that seen for GDP-bound, unpolymerized alphabeta-tubulin. Tubulins are thought to represent a distinct class of GTP-binding proteins, and conformational switching in gamma-tubulin might differ from the nucleotide-dependent switching of signalling GTPases. A crystal packing interaction replicates the lateral contacts between alpha- and beta-tubulins in the microtubule, and this association probably forms the basis for gamma-tubulin oligomerization within the gamma-TuRC. Laterally associated gamma-tubulins in the gamma-TuRC might promote microtubule nucleation by providing a template that enhances the intrinsically weak lateral interaction between alphabeta-tubulin heterodimers. Because they are dimeric, alphabeta-tubulins cannot form microtubule-like lateral associations in the curved conformation. The lateral array of gamma-tubulins we observe in the crystal reveals a unique functional property of a monomeric tubulin.

About this Structure

1Z5W is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Insights into microtubule nucleation from the crystal structure of human gamma-tubulin., Aldaz H, Rice LM, Stearns T, Agard DA, Nature. 2005 May 26;435(7041):523-7. PMID:15917813

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