1z6t
From Proteopedia
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| - | [[Image:1z6t.gif|left|200px]] | + | [[Image:1z6t.gif|left|200px]] |
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| - | '''Structure of the apoptotic protease-activating factor 1 bound to ADP''' | + | {{Structure |
| + | |PDB= 1z6t |SIZE=350|CAPTION= <scene name='initialview01'>1z6t</scene>, resolution 2.21Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structure of the apoptotic protease-activating factor 1 bound to ADP''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Z6T is a [ | + | 1Z6T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z6T OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the apoptotic protease-activating factor 1 bound to ADP., Riedl SJ, Li W, Chao Y, Schwarzenbacher R, Shi Y, Nature. 2005 Apr 14;434(7035):926-33. PMID:[http:// | + | Structure of the apoptotic protease-activating factor 1 bound to ADP., Riedl SJ, Li W, Chao Y, Schwarzenbacher R, Shi Y, Nature. 2005 Apr 14;434(7035):926-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15829969 15829969] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: nucleotide binding]] | [[Category: nucleotide binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:31:50 2008'' |
Revision as of 13:31, 20 March 2008
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| , resolution 2.21Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the apoptotic protease-activating factor 1 bound to ADP
Overview
Apoptosis is executed by caspases, which undergo proteolytic activation in response to cell death stimuli. The apoptotic protease-activating factor 1 (Apaf-1) controls caspase activation downstream of mitochondria. During apoptosis, Apaf-1 binds to cytochrome c and in the presence of ATP/dATP forms an apoptosome, leading to the recruitment and activation of the initiator caspase, caspase-9 (ref. 2). The mechanisms underlying Apaf-1 function are largely unknown. Here we report the 2.2-A crystal structure of an ADP-bound, WD40-deleted Apaf-1, which reveals the molecular mechanism by which Apaf-1 exists in an inactive state before ATP binding. The amino-terminal caspase recruitment domain packs against a three-layered alpha/beta fold, a short helical motif and a winged-helix domain, resulting in the burial of the caspase-9-binding interface. The deeply buried ADP molecule serves as an organizing centre to strengthen interactions between these four adjoining domains, thus locking Apaf-1 in an inactive conformation. Apaf-1 binds to and hydrolyses ATP/dATP and their analogues. The binding and hydrolysis of nucleotides seem to drive conformational changes that are essential for the formation of the apoptosome and the activation of caspase-9.
About this Structure
1Z6T is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the apoptotic protease-activating factor 1 bound to ADP., Riedl SJ, Li W, Chao Y, Schwarzenbacher R, Shi Y, Nature. 2005 Apr 14;434(7035):926-33. PMID:15829969
Page seeded by OCA on Thu Mar 20 15:31:50 2008
Categories: Homo sapiens | Single protein | Chao, Y. | Li, W. | Riedl, S J. | Schwarzenbacher, R. | Shi, Y. | ADP | Adp | Apaf-1 | Card | Caspase activation | Nucleotide binding
