1z96
From Proteopedia
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| - | [[Image:1z96.gif|left|200px]] | + | [[Image:1z96.gif|left|200px]] |
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| - | '''Crystal structure of the Mud1 UBA domain''' | + | {{Structure |
| + | |PDB= 1z96 |SIZE=350|CAPTION= <scene name='initialview01'>1z96</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= mud1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the Mud1 UBA domain''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Z96 is a [ | + | 1Z96 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z96 OCA]. |
==Reference== | ==Reference== | ||
| - | Mechanism of Lys48-linked polyubiquitin chain recognition by the Mud1 UBA domain., Trempe JF, Brown NR, Lowe ED, Gordon C, Campbell ID, Noble ME, Endicott JA, EMBO J. 2005 Sep 21;24(18):3178-89. Epub 2005 Sep 1. PMID:[http:// | + | Mechanism of Lys48-linked polyubiquitin chain recognition by the Mud1 UBA domain., Trempe JF, Brown NR, Lowe ED, Gordon C, Campbell ID, Noble ME, Endicott JA, EMBO J. 2005 Sep 21;24(18):3178-89. Epub 2005 Sep 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16138082 16138082] |
[[Category: Schizosaccharomyces pombe]] | [[Category: Schizosaccharomyces pombe]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ubiquitin]] | [[Category: ubiquitin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:32:40 2008'' |
Revision as of 13:32, 20 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | mud1 (Schizosaccharomyces pombe) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Mud1 UBA domain
Overview
The ubiquitin-pathway associated (UBA) domain is a 40-residue polyubiquitin-binding motif. The Schizosaccharomyces pombe protein Mud1 is an ortholog of the Saccharomyces cerevisiae DNA-damage response protein Ddi1 and binds to K48-linked polyubiquitin through its UBA domain. We have solved the crystal structure of Mud1 UBA at 1.8 angstroms resolution, revealing a canonical three-helical UBA fold. We have probed the interactions of this domain using mutagenesis, surface plasmon resonance, NMR and analytical ultracentrifugation. We show that the ubiquitin-binding surface of Mud1 UBA extends beyond previously recognized motifs and can be functionally dissected into primary and secondary ubiquitin-binding sites. Mutation of Phe330 to alanine, a residue exposed between helices 2 and 3, significantly reduces the affinity of the Mud1 UBA domain for K48-linked polyubiquitin, despite leaving the primary binding surface functionally intact. Moreover, K48-linked diubiquitin binds a single Mud1 UBA domain even in the presence of excess UBA. We therefore propose a mechanism for the recognition of K48-linked polyubiquitin chains by Mud1 in which diubiquitin units are specifically recognized by a single UBA domain.
About this Structure
1Z96 is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.
Reference
Mechanism of Lys48-linked polyubiquitin chain recognition by the Mud1 UBA domain., Trempe JF, Brown NR, Lowe ED, Gordon C, Campbell ID, Noble ME, Endicott JA, EMBO J. 2005 Sep 21;24(18):3178-89. Epub 2005 Sep 1. PMID:16138082
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