1z9b
From Proteopedia
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| - | [[Image:1z9b.gif|left|200px]] | + | [[Image:1z9b.gif|left|200px]] |
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| - | '''Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2''' | + | {{Structure |
| + | |PDB= 1z9b |SIZE=350|CAPTION= <scene name='initialview01'>1z9b</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= infB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Z9B is a [ | + | 1Z9B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z9B OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2., Wienk H, Tomaselli S, Bernard C, Spurio R, Picone D, Gualerzi CO, Boelens R, Protein Sci. 2005 Sep;14(9):2461-8. Epub 2005 Aug 4. PMID:[http:// | + | Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2., Wienk H, Tomaselli S, Bernard C, Spurio R, Picone D, Gualerzi CO, Boelens R, Protein Sci. 2005 Sep;14(9):2461-8. Epub 2005 Aug 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16081655 16081655] |
[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein synthesis translation intiation if2 fmet-trna nmr structure]] | [[Category: protein synthesis translation intiation if2 fmet-trna nmr structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:32:43 2008'' |
Revision as of 13:32, 20 March 2008
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| Gene: | infB (Geobacillus stearothermophilus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2
Overview
IF2 is one of three bacterial translation initiation factors that are conserved through all kingdoms of life. It binds the 30S and 50S ribosomal subunits, as well as fMet-tRNAf(Met). After these interactions, fMet-tRNAf(Met) is oriented to the ribosomal P-site where the first amino acid of the nascent polypeptide, formylmethionine, is presented. The C-terminal domain of Bacillus stearothermophilus IF2, which is responsible for recognition and binding of fMet-tRNAf(Met), contains two structured modules. Previously, the solution structure of the most C-terminal module, IF2-C2, has been elucidated by NMR spectroscopy and direct interactions between this subdomain and fMet-tRNAf(Met) were reported. In the present NMR study we have obtained the spectral assignment of the other module of the C-terminal domain (IF2-C1) and determined its solution structure and backbone dynamics. The IF2-C1 core forms a flattened fold consisting of a central four-stranded parallel beta-sheet flanked by three alpha-helices. Although its overall organization resembles that of subdomain III of the archaeal IF2-homolog eIF5B whose crystal structure had previously been reported, some differences of potential functional significance are evident.
About this Structure
1Z9B is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2., Wienk H, Tomaselli S, Bernard C, Spurio R, Picone D, Gualerzi CO, Boelens R, Protein Sci. 2005 Sep;14(9):2461-8. Epub 2005 Aug 4. PMID:16081655
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