1zem
From Proteopedia
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| - | [[Image:1zem.gif|left|200px]] | + | [[Image:1zem.gif|left|200px]] |
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| - | '''Crystal Structure of NAD+-Bound Xylitol Dehydrogenase''' | + | {{Structure |
| + | |PDB= 1zem |SIZE=350|CAPTION= <scene name='initialview01'>1zem</scene>, resolution 1.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/D-xylulose_reductase D-xylulose reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.9 1.1.1.9] | ||
| + | |GENE= AB091690 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=442 Gluconobacter oxydans]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of NAD+-Bound Xylitol Dehydrogenase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZEM is a [ | + | 1ZEM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gluconobacter_oxydans Gluconobacter oxydans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZEM OCA]. |
==Reference== | ==Reference== | ||
| - | Structure-guided engineering of xylitol dehydrogenase cosubstrate specificity., Ehrensberger AH, Elling RA, Wilson DK, Structure. 2006 Mar;14(3):567-75. PMID:[http:// | + | Structure-guided engineering of xylitol dehydrogenase cosubstrate specificity., Ehrensberger AH, Elling RA, Wilson DK, Structure. 2006 Mar;14(3):567-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16531240 16531240] |
[[Category: D-xylulose reductase]] | [[Category: D-xylulose reductase]] | ||
[[Category: Gluconobacter oxydans]] | [[Category: Gluconobacter oxydans]] | ||
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[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:34:31 2008'' |
Revision as of 13:34, 20 March 2008
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| , resolution 1.9Å | |||||||
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| Ligands: | and | ||||||
| Gene: | AB091690 (Gluconobacter oxydans) | ||||||
| Activity: | D-xylulose reductase, with EC number 1.1.1.9 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of NAD+-Bound Xylitol Dehydrogenase
Overview
Xylitol dehydrogenase (XDH) is one of several enzymes responsible for assimilating xylose into eukaryotic metabolism and is useful for fermentation of xylose contained in agricultural byproducts to produce ethanol. For efficient xylose utilization at high flux rates, cosubstrates should be recycled between the NAD+-specific XDH and the NADPH-preferring xylose reductase, another enzyme in the pathway. To understand and alter the cosubstrate specificity of XDH, we determined the crystal structure of the Gluconobacter oxydans holoenzyme to 1.9 angstroms resolution. The structure reveals that NAD+ specificity is largely conferred by Asp38, which interacts with the hydroxyls of the adenosine ribose. Met39 stacked under the purine ring and was also located near the 2' hydroxyl. Based on the location of these residues and on sequence alignments with related enzymes of various cosubstrate specificities, we constructed a double mutant (D38S/M39R) that was able to exclusively use NADP+, with no loss of activity.
About this Structure
1ZEM is a Single protein structure of sequence from Gluconobacter oxydans. Full crystallographic information is available from OCA.
Reference
Structure-guided engineering of xylitol dehydrogenase cosubstrate specificity., Ehrensberger AH, Elling RA, Wilson DK, Structure. 2006 Mar;14(3):567-75. PMID:16531240
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