1zh6
From Proteopedia
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| - | [[Image:1zh6.gif|left|200px]] | + | [[Image:1zh6.gif|left|200px]] |
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| - | '''Crystal Structure of p-acetylphenylalanine-tRNA synthetase in complex with p-acetylphenylalanine''' | + | {{Structure |
| + | |PDB= 1zh6 |SIZE=350|CAPTION= <scene name='initialview01'>1zh6</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=4AF:4-ACETYL-L-PHENYLALANINE'>4AF</scene> and <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] | ||
| + | |GENE= tyrS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of p-acetylphenylalanine-tRNA synthetase in complex with p-acetylphenylalanine''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZH6 is a [ | + | 1ZH6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZH6 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural characterization of a p-acetylphenylalanyl aminoacyl-tRNA synthetase., Turner JM, Graziano J, Spraggon G, Schultz PG, J Am Chem Soc. 2005 Nov 2;127(43):14976-7. PMID:[http:// | + | Structural characterization of a p-acetylphenylalanyl aminoacyl-tRNA synthetase., Turner JM, Graziano J, Spraggon G, Schultz PG, J Am Chem Soc. 2005 Nov 2;127(43):14976-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16248607 16248607] |
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: unnatural amino acid]] | [[Category: unnatural amino acid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:35:26 2008'' |
Revision as of 13:35, 20 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | tyrS (Methanocaldococcus jannaschii) | ||||||
| Activity: | Tyrosine--tRNA ligase, with EC number 6.1.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of p-acetylphenylalanine-tRNA synthetase in complex with p-acetylphenylalanine
Overview
It has been recently shown that orthogonal tRNA/aminoacyl-tRNA synthetase pairs can be evolved to allow genetic incorporation of unnatural amino acids into proteins in both prokaryotes and eukaryotes. Here we describe the crystal structure of an evolved aminoacyl-tRNA synthetase that charges the unnatural amino acid p-acetylphenylalanine. Molecular recognition is due to altered hydrogen bonding and packing interactions with bound substrate that result from changes in both side-chain and backbone conformation.
About this Structure
1ZH6 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
Structural characterization of a p-acetylphenylalanyl aminoacyl-tRNA synthetase., Turner JM, Graziano J, Spraggon G, Schultz PG, J Am Chem Soc. 2005 Nov 2;127(43):14976-7. PMID:16248607
Page seeded by OCA on Thu Mar 20 15:35:26 2008
