1zla
From Proteopedia
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| - | [[Image:1zla.gif|left|200px]] | + | [[Image:1zla.gif|left|200px]] |
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| - | '''X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core''' | + | {{Structure |
| + | |PDB= 1zla |SIZE=350|CAPTION= <scene name='initialview01'>1zla</scene>, resolution 2.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Alpha-Satellite DNA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), Histone H3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), Histone H4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), Histone H2A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=89961 Expression vector pCY215]), Histone H2B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]) | ||
| + | }} | ||
| + | |||
| + | '''X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZLA is a [ | + | 1ZLA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Expression_vector_pcy215 Expression vector pcy215], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLA OCA]. |
==Reference== | ==Reference== | ||
| - | The nucleosomal surface as a docking station for Kaposi's sarcoma herpesvirus LANA., Barbera AJ, Chodaparambil JV, Kelley-Clarke B, Joukov V, Walter JC, Luger K, Kaye KM, Science. 2006 Feb 10;311(5762):856-61. PMID:[http:// | + | The nucleosomal surface as a docking station for Kaposi's sarcoma herpesvirus LANA., Barbera AJ, Chodaparambil JV, Kelley-Clarke B, Joukov V, Walter JC, Luger K, Kaye KM, Science. 2006 Feb 10;311(5762):856-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16469929 16469929] |
[[Category: Expression vector pcy215]] | [[Category: Expression vector pcy215]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: protein/protein interaction]] | [[Category: protein/protein interaction]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:36:53 2008'' |
Revision as of 13:36, 20 March 2008
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| , resolution 2.9Å | |||||||
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| Gene: | Alpha-Satellite DNA (Homo sapiens), Histone H3 (Xenopus laevis), Histone H4 (Xenopus laevis), Histone H2A (Expression vector pCY215), Histone H2B (Xenopus laevis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core
Overview
Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) mediates viral genome attachment to mitotic chromosomes. We find that N-terminal LANA docks onto chromosomes by binding nucleosomes through the folded region of histones H2A-H2B. The same LANA residues were required for both H2A-H2B binding and chromosome association. Further, LANA did not bind Xenopus sperm chromatin, which is deficient in H2A-H2B; chromatin binding was rescued after assembly of nucleosomes containing H2A-H2B. We also describe the 2.9-angstrom crystal structure of a nucleosome complexed with the first 23 LANA amino acids. The LANA peptide forms a hairpin that interacts exclusively with an acidic H2A-H2B region that is implicated in the formation of higher order chromatin structure. Our findings present a paradigm for how nucleosomes may serve as binding platforms for viral and cellular proteins and reveal a previously unknown mechanism for KSHV latency.
About this Structure
1ZLA is a Protein complex structure of sequences from Expression vector pcy215, Homo sapiens and Xenopus laevis. Full crystallographic information is available from OCA.
Reference
The nucleosomal surface as a docking station for Kaposi's sarcoma herpesvirus LANA., Barbera AJ, Chodaparambil JV, Kelley-Clarke B, Joukov V, Walter JC, Luger K, Kaye KM, Science. 2006 Feb 10;311(5762):856-61. PMID:16469929
Page seeded by OCA on Thu Mar 20 15:36:53 2008
Categories: Expression vector pcy215 | Homo sapiens | Protein complex | Xenopus laevis | Barbera, A J. | Chodaparambil, J V. | Kaye, K M. | Luger, K. | Chromatin | Kaposi's sarcoma herpes virus (kshv) | Latency associated nuclear antigen (lana) | Nucleosome core particle | Protein/protein interaction
