1zme
From Proteopedia
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| - | [[Image:1zme.gif|left|200px]] | + | [[Image:1zme.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF PUT3/DNA COMPLEX''' | + | {{Structure |
| + | |PDB= 1zme |SIZE=350|CAPTION= <scene name='initialview01'>1zme</scene>, resolution 2.5Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF PUT3/DNA COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZME is a [ | + | 1ZME is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZME OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a PUT3-DNA complex reveals a novel mechanism for DNA recognition by a protein containing a Zn2Cys6 binuclear cluster., Swaminathan K, Flynn P, Reece RJ, Marmorstein R, Nat Struct Biol. 1997 Sep;4(9):751-9. PMID:[http:// | + | Crystal structure of a PUT3-DNA complex reveals a novel mechanism for DNA recognition by a protein containing a Zn2Cys6 binuclear cluster., Swaminathan K, Flynn P, Reece RJ, Marmorstein R, Nat Struct Biol. 1997 Sep;4(9):751-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9303004 9303004] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: zn2cys6]] | [[Category: zn2cys6]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:37:16 2008'' |
Revision as of 13:37, 20 March 2008
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| , resolution 2.5Å | |||||||
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| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF PUT3/DNA COMPLEX
Overview
PUT3 is a member of a family of at least 79 fungal transcription factors that contain a six-cysteine, two-zinc domain called a 'Zn2Cys6 binuclear cluster'. We have determined the crystal structure of the DNA binding region from the PUT3 protein bound to its cognate DNA target. The structure reveals that the PUT3 homodimer is bound asymmetrically to the DNA site. This asymmetry orients a beta-strand from one protein subunit into the minor groove of the DNA resulting in a partial amino acid-base pair intercalation and extensive direct and water-mediated protein interactions with the minor groove of the DNA. These interactions facilitate a sequence dependent kink at the centre of the DNA site and specify the intervening base pairs separating two DNA half-sites that are contacted in the DNA major groove. A comparison with the GAL4-DNA and PPR1-DNA complexes shows how a family of related DNA binding proteins can use a diverse set of mechanisms to discriminate between the base pairs separating conserved DNA half-sites.
About this Structure
1ZME is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of a PUT3-DNA complex reveals a novel mechanism for DNA recognition by a protein containing a Zn2Cys6 binuclear cluster., Swaminathan K, Flynn P, Reece RJ, Marmorstein R, Nat Struct Biol. 1997 Sep;4(9):751-9. PMID:9303004
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