1znn
From Proteopedia
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| - | [[Image:1znn.gif|left|200px]] | + | [[Image:1znn.gif|left|200px]] |
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| - | '''Structure of the synthase subunit of PLP synthase''' | + | {{Structure |
| + | |PDB= 1znn |SIZE=350|CAPTION= <scene name='initialview01'>1znn</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structure of the synthase subunit of PLP synthase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZNN is a [ | + | 1ZNN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZNN OCA]. |
==Reference== | ==Reference== | ||
| - | A new arrangement of (beta/alpha)8 barrels in the synthase subunit of PLP synthase., Zhu J, Burgner JW, Harms E, Belitsky BR, Smith JL, J Biol Chem. 2005 Jul 29;280(30):27914-23. Epub 2005 May 23. PMID:[http:// | + | A new arrangement of (beta/alpha)8 barrels in the synthase subunit of PLP synthase., Zhu J, Burgner JW, Harms E, Belitsky BR, Smith JL, J Biol Chem. 2005 Jul 29;280(30):27914-23. Epub 2005 May 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15911615 15911615] |
[[Category: Geobacillus stearothermophilus]] | [[Category: Geobacillus stearothermophilus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:37:43 2008'' |
Revision as of 13:37, 20 March 2008
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| , resolution 2.20Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the synthase subunit of PLP synthase
Overview
Pyridoxal 5'-phosphate (PLP, vitamin B6), a cofactor in many enzymatic reactions, has two distinct biosynthetic routes, which do not coexist in any organism. Two proteins, known as PdxS and PdxT, together form a PLP synthase in plants, fungi, archaea, and some eubacteria. PLP synthase is a heteromeric glutamine amidotransferase in which PdxT produces ammonia from glutamine and PdxS combines ammonia with five- and three-carbon phosphosugars to form PLP. In the 2.2-A crystal structure, PdxS is a cylindrical dodecamer of subunits having the classic (beta/alpha)8 barrel fold. PdxS subunits form two hexameric rings with the active sites positioned on the inside. The hexamer and dodecamer forms coexist in solution. A novel phosphate-binding site is suggested by bound sulfate. The sulfate and another bound molecule, methyl pentanediol, were used to model the substrate ribulose 5-phosphate, and to propose catalytic roles for residues in the active site. The distribution of conserved surfaces in the PdxS dodecamer was used to predict a docking site for the glutaminase partner, PdxT.
About this Structure
1ZNN is a Protein complex structure of sequences from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
A new arrangement of (beta/alpha)8 barrels in the synthase subunit of PLP synthase., Zhu J, Burgner JW, Harms E, Belitsky BR, Smith JL, J Biol Chem. 2005 Jul 29;280(30):27914-23. Epub 2005 May 23. PMID:15911615
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