4gsj

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[[Image:4gsj.png|left|200px]]
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==Crystal structure of Atg7 NTD K14A F16A D18A mutant==
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<StructureSection load='4gsj' size='340' side='right' caption='[[4gsj]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4gsj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GSJ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gsk|4gsk]], [[4gsl|4gsl]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">APG7, ATG7, CVT2, YHR171W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gsj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gsj RCSB], [http://www.ebi.ac.uk/pdbsum/4gsj PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/ATG7_YEAST ATG7_YEAST]] E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes.<ref>PMID:9759731</ref> <ref>PMID:8224160</ref> <ref>PMID:7593182</ref> <ref>PMID:10233148</ref> <ref>PMID:10233150</ref> <ref>PMID:11100732</ref> <ref>PMID:11149920</ref> <ref>PMID:12965207</ref> <ref>PMID:15277523</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Core functions of autophagy are mediated by ubiquitin-like protein (UBL) cascades, in which a homodimeric E1 enzyme, Atg7, directs the UBLs Atg8 and Atg12 to their respective E2 enzymes, Atg3 and Atg10. Crystallographic and mutational analyses of yeast (Atg7-Atg3)(2) and (Atg7-Atg10)(2) complexes reveal noncanonical, multisite E1-E2 recognition in autophagy. Atg7's unique N-terminal domain recruits distinctive elements from the Atg3 and Atg10 'backsides'. This, along with E1 and E2 conformational variability, allows presentation of 'frontside' Atg3 and Atg10 active sites to the catalytic cysteine in the C-terminal domain from the opposite Atg7 protomer in the homodimer. Despite different modes of binding, the data suggest that common principles underlie conjugation in both noncanonical and canonical UBL cascades, whereby flexibly tethered E1 domains recruit E2s through surfaces remote from their active sites to juxtapose the E1 and E2 catalytic cysteines.
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{{STRUCTURE_4gsj| PDB=4gsj | SCENE= }}
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Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures.,Kaiser SE, Mao K, Taherbhoy AM, Yu S, Olszewski JL, Duda DM, Kurinov I, Deng A, Fenn TD, Klionsky DJ, Schulman BA Nat Struct Mol Biol. 2012 Nov 11. doi: 10.1038/nsmb.2415. PMID:23142976<ref>PMID:23142976</ref>
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===Crystal structure of Atg7 NTD K14A F16A D18A mutant===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_23142976}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[4gsj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSJ OCA].
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</StructureSection>
[[Category: Saccharomyces cerevisiae s288c]]
[[Category: Saccharomyces cerevisiae s288c]]
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[[Category: Deng, A.]]
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[[Category: Deng, A]]
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[[Category: Duda, D M.]]
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[[Category: Duda, D M]]
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[[Category: Fenn, T D.]]
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[[Category: Fenn, T D]]
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[[Category: Kaiser, S E.]]
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[[Category: Kaiser, S E]]
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[[Category: Klionsky, D J.]]
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[[Category: Klionsky, D J]]
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[[Category: Kurinov, I.]]
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[[Category: Kurinov, I]]
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[[Category: Mao, K.]]
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[[Category: Mao, K]]
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[[Category: Olszewski, J L.]]
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[[Category: Olszewski, J L]]
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[[Category: Schulman, B A.]]
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[[Category: Schulman, B A]]
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[[Category: Taherbhoy, A M.]]
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[[Category: Taherbhoy, A M]]
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[[Category: Yu, S.]]
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[[Category: Yu, S]]
[[Category: Protein transport]]
[[Category: Protein transport]]
[[Category: Ubiquitin-like protein activation enzyme]]
[[Category: Ubiquitin-like protein activation enzyme]]

Revision as of 20:42, 24 December 2014

Crystal structure of Atg7 NTD K14A F16A D18A mutant

4gsj, resolution 1.69Å

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