1zq1
From Proteopedia
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| - | [[Image:1zq1.gif|left|200px]] | + | [[Image:1zq1.gif|left|200px]] |
| - | + | ||
| - | '''Structure of GatDE tRNA-Dependent Amidotransferase from Pyrococcus abyssi''' | + | {{Structure |
| + | |PDB= 1zq1 |SIZE=350|CAPTION= <scene name='initialview01'>1zq1</scene>, resolution 3.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ASP:ASPARTIC ACID'>ASP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= gatD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29292 Pyrococcus abyssi]), gatE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29292 Pyrococcus abyssi]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of GatDE tRNA-Dependent Amidotransferase from Pyrococcus abyssi''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZQ1 is a [ | + | 1ZQ1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_abyssi Pyrococcus abyssi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZQ1 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for tRNA-dependent amidotransferase function., Schmitt E, Panvert M, Blanquet S, Mechulam Y, Structure. 2005 Oct;13(10):1421-33. PMID:[http:// | + | Structural basis for tRNA-dependent amidotransferase function., Schmitt E, Panvert M, Blanquet S, Mechulam Y, Structure. 2005 Oct;13(10):1421-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16216574 16216574] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Pyrococcus abyssi]] | [[Category: Pyrococcus abyssi]] | ||
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[[Category: x-ray; 3d structure; asparaginase 1 family; gatd subfamily]] | [[Category: x-ray; 3d structure; asparaginase 1 family; gatd subfamily]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:38:27 2008'' |
Revision as of 13:38, 20 March 2008
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| , resolution 3.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | gatD (Pyrococcus abyssi), gatE (Pyrococcus abyssi) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of GatDE tRNA-Dependent Amidotransferase from Pyrococcus abyssi
Overview
Besides direct charging of tRNAs by aminoacyl-tRNA synthetases, indirect routes also ensure attachment of some amino acids onto tRNA. Such routes may explain how new amino acids entered into protein synthesis. In archaea and in most bacteria, tRNA(Gln) is first misaminoacylated by glutamyl-tRNA synthetase. Glu-tRNA(Gln) is then matured into Gln-tRNA(Gln) by a tRNA-dependent amidotransferase. We report the structure of a tRNA-dependent amidotransferase-that of GatDE from Pyrococcus abyssi. The 3.0 A resolution crystal structure shows a tetramer with two GatD molecules as the core and two GatE molecules at the periphery. The fold of GatE cannot be related to that of any tRNA binding enzyme. The ammonium donor site on GatD and the tRNA site on GatE are markedly distant. Comparison of GatD and L-asparaginase structures shows how the motion of a beta hairpin region containing a crucial catalytic threonine may control the overall reaction cycle of GatDE.
About this Structure
1ZQ1 is a Protein complex structure of sequences from Pyrococcus abyssi. Full crystallographic information is available from OCA.
Reference
Structural basis for tRNA-dependent amidotransferase function., Schmitt E, Panvert M, Blanquet S, Mechulam Y, Structure. 2005 Oct;13(10):1421-33. PMID:16216574
Page seeded by OCA on Thu Mar 20 15:38:27 2008
