1mc8
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1mc8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MC8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MC8 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1mc8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MC8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MC8 FirstGlance]. <br> | ||
| - | </td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mc8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mc8 RCSB], [http://www.ebi.ac.uk/pdbsum/1mc8 PDBsum]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mc8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mc8 RCSB], [http://www.ebi.ac.uk/pdbsum/1mc8 PDBsum]</span></td></tr> |
| - | <table> | + | </table> |
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/FEN_PYRHO FEN_PYRHO]] Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA (By similarity). | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Pyrococcus horikoshii]] | [[Category: Pyrococcus horikoshii]] | ||
| - | [[Category: Abe, J | + | [[Category: Abe, J]] |
| - | [[Category: Harata, K | + | [[Category: Harata, K]] |
| - | [[Category: Matsui, E | + | [[Category: Matsui, E]] |
| - | [[Category: Matsui, I | + | [[Category: Matsui, I]] |
| - | [[Category: Musti, K V | + | [[Category: Musti, K V]] |
| - | [[Category: Yamazaki, K | + | [[Category: Yamazaki, K]] |
[[Category: Flexible loop]] | [[Category: Flexible loop]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
Revision as of 21:13, 24 December 2014
Crystal Structure of Flap Endonuclease-1 R42E mutant from Pyrococcus horikoshii
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