3zpv
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure of Drosophila Pygo PHD finger in complex with Legless HD1 domain== | |
| - | + | <StructureSection load='3zpv' size='340' side='right' caption='[[3zpv]], [[Resolution|resolution]] 2.68Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3zpv]] is a 36 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZPV FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zpv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zpv RCSB], [http://www.ebi.ac.uk/pdbsum/3zpv PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/BCL9_DROME BCL9_DROME]] Involved in signal transduction through the Wnt pathway. [[http://www.uniprot.org/uniprot/PYGO_DROME PYGO_DROME]] Involved in signal transduction through the Wnt pathway. | [[http://www.uniprot.org/uniprot/BCL9_DROME BCL9_DROME]] Involved in signal transduction through the Wnt pathway. [[http://www.uniprot.org/uniprot/PYGO_DROME PYGO_DROME]] Involved in signal transduction through the Wnt pathway. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pygo proteins promote Armadillo- and beta-catenin-dependent transcription, by relieving Groucho-dependent repression of Wnt targets. Their PHD fingers bind histone H3 tail methylated at lysine 4, and to the HD1 domain of their Legless/BCL9 cofactors, linking Pygo to Armadillo/beta-catenin. Intriguingly, fly Pygo orthologs exhibit a tryptophan > phenylalanine substitution in their histone pocket-divider which reduces their affinity for histones. Here, we use X-ray crystallography and NMR, to discover a conspicuous groove bordering this phenylalanine in the Drosophila PHD-HD1 complex-a semi-aromatic cage recognizing asymmetrically methylated arginine 2 (R2me2a), a chromatin mark of silenced genes. Our structural model of the ternary complex reveals a distinct mode of dimethylarginine recognition, involving a polar interaction between R2me2a and its groove, the structural integrity of which is crucial for normal tissue patterning. Notably, humanized fly Pygo derepresses Notch targets, implying an inherent Notch-related function of classical Pygo orthologs, disabled in fly Pygo, which thus appears dedicated to Wnt signaling. | ||
| - | + | Evolutionary Adaptation of the Fly Pygo PHD Finger toward Recognizing Histone H3 Tail Methylated at Arginine 2.,Miller TC, Mieszczanek J, Sanchez-Barrena MJ, Rutherford TJ, Fiedler M, Bienz M Structure. 2013 Oct 30. pii: S0969-2126(13)00364-X. doi:, 10.1016/j.str.2013.09.013. PMID:24183574<ref>PMID:24183574</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Drome]] | [[Category: Drome]] | ||
| - | [[Category: Bienz, M | + | [[Category: Bienz, M]] |
| - | [[Category: Fiedler, M | + | [[Category: Fiedler, M]] |
| - | [[Category: Mieszczanek, J | + | [[Category: Mieszczanek, J]] |
| - | [[Category: Miller, T C.R | + | [[Category: Miller, T C.R]] |
| - | [[Category: Rutherford, T J | + | [[Category: Rutherford, T J]] |
| - | [[Category: Sanchez-Barrena, M J | + | [[Category: Sanchez-Barrena, M J]] |
[[Category: Histone h3 tail binding]] | [[Category: Histone h3 tail binding]] | ||
[[Category: Transcription]] | [[Category: Transcription]] | ||
[[Category: Wnt signaling pathway]] | [[Category: Wnt signaling pathway]] | ||
[[Category: Zn finger]] | [[Category: Zn finger]] | ||
Revision as of 21:29, 24 December 2014
Crystal structure of Drosophila Pygo PHD finger in complex with Legless HD1 domain
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