4pxt

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{{STRUCTURE_4pxt| PDB=4pxt | SCENE= }}
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==Structural basis for the assembly of the mitotic motor kinesin-5 into bipolar tetramers==
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===Structural basis for the assembly of the mitotic motor kinesin-5 into bipolar tetramers===
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<StructureSection load='4pxt' size='340' side='right' caption='[[4pxt]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24714498}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4pxt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PXT FirstGlance]. <br>
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==Function==
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4pxu|4pxu]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CG9191, KLP2, Klp61F, Klp61F or DmKlp61F or CG9191 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pxt RCSB], [http://www.ebi.ac.uk/pdbsum/4pxt PDBsum]</span></td></tr>
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</table>
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== Function ==
[[http://www.uniprot.org/uniprot/KL61_DROME KL61_DROME]] Important role in mitotic dividing cells. Microtubule motor required for spindle body separation. Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function.
[[http://www.uniprot.org/uniprot/KL61_DROME KL61_DROME]] Important role in mitotic dividing cells. Microtubule motor required for spindle body separation. Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Chromosome segregation during mitosis depends upon Kinesin-5 motors, which display a conserved, bipolar homotetrameric organization consisting of two motor dimers at opposite ends of a central rod. Kinesin-5 motors crosslink adjacent microtubules to drive or constrain their sliding apart, but the structural basis of their organization is unknown. In this study, we report the atomic structure of the bipolar assembly (BASS) domain that directs four Kinesin-5 subunits to form a bipolar minifilament. BASS is a novel 26-nm four-helix bundle, consisting of two anti-parallel coiled-coils at its center, stabilized by alternating hydrophobic and ionic four-helical interfaces, which based on mutagenesis experiments, are critical for tetramerization. Strikingly, N-terminal BASS helices bend as they emerge from the central bundle, swapping partner helices, to form dimeric parallel coiled-coils at both ends, which are offset by 90 degrees . We propose that BASS is a mechanically stable, plectonemically-coiled junction, transmitting forces between Kinesin-5 motor dimers during microtubule sliding. DOI: http://dx.doi.org/10.7554/eLife.02217.001.
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==About this Structure==
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Structural basis for the assembly of the mitotic motor Kinesin-5 into bipolar tetramers.,Scholey JE, Nithianantham S, Scholey JM, Al-Bassam J Elife. 2014 Apr 8;3:e02217. doi: 10.7554/eLife.02217. PMID:24714498<ref>PMID:24714498</ref>
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[[4pxt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PXT OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024714498</ref><references group="xtra"/><references/>
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</div>
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[[Category: Al-Bassam, J.]]
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== References ==
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[[Category: Nithianantham, S.]]
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<references/>
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[[Category: Scholey, J E.]]
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__TOC__
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[[Category: Scholey, J M.]]
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</StructureSection>
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[[Category: Drome]]
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[[Category: Al-Bassam, J]]
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[[Category: Nithianantham, S]]
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[[Category: Scholey, J E]]
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[[Category: Scholey, J M]]
[[Category: Anti-parallel four-helix bundle]]
[[Category: Anti-parallel four-helix bundle]]
[[Category: Bipolar assembly domain of kinesin-5]]
[[Category: Bipolar assembly domain of kinesin-5]]

Revision as of 21:48, 24 December 2014

Structural basis for the assembly of the mitotic motor kinesin-5 into bipolar tetramers

4pxt, resolution 2.90Å

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