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3rcz

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Revision as of 22:20, 24 December 2014

Rad60 SLD2 Ubc9 Complex

Structural highlights

3rcz is a 2 chain structure with sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3goe
Gene:	rad60, SPBC1921.02 (Schizosaccharomyces pombe), hus5, ubc9, SPAC30D11.13 (Schizosaccharomyces pombe)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[RAD60_SCHPO] Required for repair of DNA double strand breaks which occur during replication, or induced by UV or gamma radiation, via recombination between sister chromatids. This has a subsequent role in the maintenance of chromosome structure. May work in conjunction with the Smc5-Smc6 complex.^[1] ^[2] [UBC9_SCHPO] Catalyzes the covalent attachment of ubiquitin-like protein SUMO/Smt3 to other proteins. Required for efficient recovery from DNA damage or S-phase arrest and normal mitosis. This may be as part of a checkpoint independent recovery process.^[3] ^[4]

Publication Abstract from PubMed

Global sumoylation, SUMO chain formation and genome stabilization are all outputs generated by a limited repertoire of enzymes. Mechanisms driving selectivity for each of these processes are largely uncharacterized. Here, through crystallographic analyses we show that the SUMO E2 Ubc9 forms a non-covalent complex with a SUMO-like domain of Rad60 (SLD2). Ubc9:SLD2 and Ubc9:SUMO non-covalent complexes are structurally analogous, suggesting that differential recruitment of Ubc9 by SUMO or Rad60 provides a novel means for such selectivity. Indeed, deconvoluting Ubc9 function by disrupting either the Ubc9:SLD2 or Ubc9:SUMO non-covalent complex reveals distinct roles in facilitating sumoylation. Ubc9:SLD2 acts in the Nse2 SUMO E3 ligase-dependent pathway for DNA repair, whereas Ubc9:SUMO instead promotes global sumoylation and chain formation, via the Pli1 E3 SUMO ligase. Moreover, this Pli1-dependent SUMO chain formation causes the genome instability phenotypes of SUMO-targeted ubiquitin ligase (STUbL) mutants. Overall, we determine that unexpectedly, Ubc9 non-covalent partner choice dictates the role of sumoylation in distinct cellular pathways.

DNA Repair and Global Sumoylation are Regulated by Distinct Ubc9 Non-covalent Complexes.,Prudden J, Perry JJ, Nie M, Vashisht AA, Arvai AS, Hitomi C, Guenther G, Wohlschlegel JA, Tainer JA, Boddy MN Mol Cell Biol. 2011 Mar 28. PMID:21444718^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Morishita T, Tsutsui Y, Iwasaki H, Shinagawa H. The Schizosaccharomyces pombe rad60 gene is essential for repairing double-strand DNA breaks spontaneously occurring during replication and induced by DNA-damaging agents. Mol Cell Biol. 2002 May;22(10):3537-48. PMID:11971984
↑ Boddy MN, Shanahan P, McDonald WH, Lopez-Girona A, Noguchi E, Yates III JR, Russell P. Replication checkpoint kinase Cds1 regulates recombinational repair protein Rad60. Mol Cell Biol. 2003 Aug;23(16):5939-46. PMID:12897162
↑ Ho JC, Watts FZ. Characterization of SUMO-conjugating enzyme mutants in Schizosaccharomyces pombe identifies a dominant-negative allele that severely reduces SUMO conjugation. Biochem J. 2003 May 15;372(Pt 1):97-104. PMID:12597774 doi:http://dx.doi.org/10.1042/BJ20021645
↑ al-Khodairy F, Enoch T, Hagan IM, Carr AM. The Schizosaccharomyces pombe hus5 gene encodes a ubiquitin conjugating enzyme required for normal mitosis. J Cell Sci. 1995 Feb;108 ( Pt 2):475-86. PMID:7768995
↑ Prudden J, Perry JJ, Nie M, Vashisht AA, Arvai AS, Hitomi C, Guenther G, Wohlschlegel JA, Tainer JA, Boddy MN. DNA Repair and Global Sumoylation are Regulated by Distinct Ubc9 Non-covalent Complexes. Mol Cell Biol. 2011 Mar 28. PMID:21444718 doi:10.1128/MCB.05188-11

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3rcz"

@@ Line 7: / Line 7: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rcz RCSB], [http://www.ebi.ac.uk/pdbsum/3rcz PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/RAD60_SCHPO RAD60_SCHPO]] Required for repair of DNA double strand breaks which occur during replication, or induced by UV or gamma radiation, via recombination between sister chromatids. This has a subsequent role in the maintenance of chromosome structure. May work in conjunction with the Smc5-Smc6 complex.<ref>PMID:11971984</ref> <ref>PMID:12897162</ref>  [[http://www.uniprot.org/uniprot/UBC9_SCHPO UBC9_SCHPO]] Catalyzes the covalent attachment of ubiquitin-like protein SUMO/Smt3 to other proteins. Required for efficient recovery from DNA damage or S-phase arrest and normal mitosis. This may be as part of a checkpoint independent recovery process.<ref>PMID:12597774</ref> <ref>PMID:7768995</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

3rcz

From Proteopedia

Revision as of 22:20, 24 December 2014

Rad60 SLD2 Ubc9 Complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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