283d

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[[Image:283d.jpg|left|200px]]<br /><applet load="283d" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:283d.jpg|left|200px]]
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caption="283d, resolution 2.300&Aring;" />
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'''A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING'''<br />
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{{Structure
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|PDB= 283d |SIZE=350|CAPTION= <scene name='initialview01'>283d</scene>, resolution 2.300&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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283D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=283D OCA].
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283D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=283D OCA].
==Reference==
==Reference==
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A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8917508 8917508]
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A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8917508 8917508]
[[Category: ]]
[[Category: ]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: unusual rna]]
[[Category: unusual rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:43:36 2008''

Revision as of 13:43, 20 March 2008


PDB ID 283d

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, resolution 2.300Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING


Overview

The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.

About this Structure

283D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:8917508 [[Category: ]]

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