296d

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[[Image:296d.gif|left|200px]]<br /><applet load="296d" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:296d.gif|left|200px]]
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caption="296d, resolution 2.250&Aring;" />
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'''SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX'''<br />
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{{Structure
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|PDB= 296d |SIZE=350|CAPTION= <scene name='initialview01'>296d</scene>, resolution 2.250&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=HT:2'-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI-BENZIMIDAZOLE'>HT</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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296D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=HT:'>HT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA].
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296D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA].
==Reference==
==Reference==
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Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7515488 7515488]
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Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7515488 7515488]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Brown, D G.]]
[[Category: Brown, D G.]]
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[[Category: double helix]]
[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:43:46 2008''

Revision as of 13:43, 20 March 2008

Image:296d.gif


PDB ID 296d

Drag the structure with the mouse to rotate
, resolution 2.250Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX


Overview

The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width.

About this Structure

296D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:7515488

Page seeded by OCA on Thu Mar 20 15:43:46 2008

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