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299d

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[[Image:299d.jpg|left|200px]]<br /><applet load="299d" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:299d.jpg|left|200px]]
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caption="299d, resolution 3.000&Aring;" />
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'''CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: THE HAMMERHEAD RIBOZYME'''<br />
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{{Structure
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|PDB= 299d |SIZE=350|CAPTION= <scene name='initialview01'>299d</scene>, resolution 3.000&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: THE HAMMERHEAD RIBOZYME'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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299D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=299D OCA].
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299D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=299D OCA].
==Reference==
==Reference==
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Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme., Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A, Science. 1996 Dec 20;274(5295):2065-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8953035 8953035]
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Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme., Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A, Science. 1996 Dec 20;274(5295):2065-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8953035 8953035]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Arnold, J R.P.]]
[[Category: Arnold, J R.P.]]
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[[Category: rna hammerhead ribozyme]]
[[Category: rna hammerhead ribozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:43:51 2008''

Revision as of 13:43, 20 March 2008

Image:299d.jpg


PDB ID 299d

Drag the structure with the mouse to rotate
, resolution 3.000Å
Coordinates: save as pdb, mmCIF, xml



CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: THE HAMMERHEAD RIBOZYME


Overview

The crystal structure of an unmodified hammerhead RNA in the absence of divalent metal ions has been solved, and it was shown that this ribozyme can cleave itself in the crystal when divalent metal ions are added. This biologically active RNA fold is the same as that found previously for two modified hammerhead ribozymes. Addition of divalent cations at low pH makes it possible to capture the uncleaved RNA in metal-bound form. A conformational intermediate, having an additional Mg(II) bound to the cleavage-site phosphate, was captured by freeze-trapping the RNA at an active pH prior to cleavage. The most significant conformational changes were limited to the active site of the ribozyme, and the changed conformation requires only small additional movements to reach a proposed transition-state.

About this Structure

299D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme., Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A, Science. 1996 Dec 20;274(5295):2065-9. PMID:8953035

Page seeded by OCA on Thu Mar 20 15:43:51 2008

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