4nrl

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{{STRUCTURE_4nrl| PDB=4nrl | SCENE= }}
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==Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40==
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===Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40===
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<StructureSection load='4nrl' size='340' side='right' caption='[[4nrl]], [[Resolution|resolution]] 2.72&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24503069}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4nrl]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Inble Inble]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NRL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NRL FirstGlance]. <br>
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==Function==
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nrj|4nrj]], [[4nrk|4nrk]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=107412 INBLE])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nrl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nrl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nrl RCSB], [http://www.ebi.ac.uk/pdbsum/4nrl PDBsum]</span></td></tr>
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</table>
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== Function ==
[[http://www.uniprot.org/uniprot/HEMA_INBLE HEMA_INBLE]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
[[http://www.uniprot.org/uniprot/HEMA_INBLE HEMA_INBLE]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Diverged ~4000 years ago, influenza B virus has several important differences from influenza A virus, including lower receptor-binding affinity and highly restricted host range. Based on our prior structural studies, we hypothesized that a single-residue difference in the receptor-binding site of hemagglutinin (HA), Phe-95 in influenza B virus versus Tyr-98 in influenza A/H1-H15, is possibly a key determinant for the low receptor-binding affinity. Here we demonstrate that the mutation Phe95--&gt;Tyr in influenza B virus HA restores all three hydrogen bonds made by Tyr-98 in influenza A/H1-15 HA and has the potential to enhance receptor binding. However, the full realization of this potential is influenced by the local environment into which the mutation is introduced. The binding and replication of the recombinant viruses correlate well with the receptor-binding capabilities of HA. These results are discussed in relation to the roles of Phe-95 in receptor binding and pathogenicity of influenza B virus.
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The roles of hemagglutinin Phe-95 in receptor binding and pathogenicity of influenza B virus.,Ni F, Nnadi Mbawuike I, Kondrashkina E, Wang Q Virology. 2014 Feb;450-451:71-83. doi: 10.1016/j.virol.2013.11.038. Epub 2013 Dec, 22. PMID:24503069<ref>PMID:24503069</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4nrl]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NRL OCA].
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</div>
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==Reference==
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==See Also==
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<ref group="xtra">PMID:024503069</ref><references group="xtra"/><references/>
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*[[Hemagglutinin|Hemagglutinin]]
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[[Category: Kondrashkina, E.]]
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== References ==
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[[Category: Mbawuike, I N.]]
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<references/>
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[[Category: Ni, F.]]
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__TOC__
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[[Category: Wang, Q.]]
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</StructureSection>
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[[Category: Inble]]
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[[Category: Kondrashkina, E]]
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[[Category: Mbawuike, I N]]
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[[Category: Ni, F]]
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[[Category: Wang, Q]]
[[Category: Ha]]
[[Category: Ha]]
[[Category: Viral protein]]
[[Category: Viral protein]]

Revision as of 22:47, 24 December 2014

Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40

4nrl, resolution 2.72Å

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