2a3r

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[[Image:2a3r.gif|left|200px]]<br /><applet load="2a3r" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2a3r.gif|left|200px]]
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caption="2a3r, resolution 2.60&Aring;" />
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'''Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate'''<br />
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{{Structure
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|PDB= 2a3r |SIZE=350|CAPTION= <scene name='initialview01'>2a3r</scene>, resolution 2.60&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=A3P:ADENOSINE-3'-5'-DIPHOSPHATE'>A3P</scene> and <scene name='pdbligand=LDP:L-DOPAMINE'>LDP</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1]
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|GENE=
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}}
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'''Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2A3R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=A3P:'>A3P</scene> and <scene name='pdbligand=LDP:'>LDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3R OCA].
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2A3R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3R OCA].
==Reference==
==Reference==
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Crystal structure of human sulfotransferase SULT1A3 in complex with dopamine and 3'-phosphoadenosine 5'-phosphate., Lu JH, Li HT, Liu MC, Zhang JP, Li M, An XM, Chang WR, Biochem Biophys Res Commun. 2005 Sep 23;335(2):417-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16083857 16083857]
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Crystal structure of human sulfotransferase SULT1A3 in complex with dopamine and 3'-phosphoadenosine 5'-phosphate., Lu JH, Li HT, Liu MC, Zhang JP, Li M, An XM, Chang WR, Biochem Biophys Res Commun. 2005 Sep 23;335(2):417-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16083857 16083857]
[[Category: Aryl sulfotransferase]]
[[Category: Aryl sulfotransferase]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: sult1a3]]
[[Category: sult1a3]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:45:04 2008''

Revision as of 13:45, 20 March 2008

Image:2a3r.gif


PDB ID 2a3r

Drag the structure with the mouse to rotate
, resolution 2.60Å
Ligands: and
Activity: Aryl sulfotransferase, with EC number 2.8.2.1
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate


Contents

Overview

The human sulfotransferase, SULT1A3, catalyzes specifically the sulfonation of monoamines such as dopamine, epinephrine, and norepinephrine. SULT1A3 also has a unique 3,4-dihydroxyphenylalanine (Dopa)/tyrosine-sulfating activity that is preferentially toward their D-form enantiomers and can be stimulated dramatically by Mn2+. To further our understanding of the molecular basis for the unique substrate specificity of this enzyme, we solved the crystal structure of human SULT1A3, complexed with dopamine and 3'-phosphoadenosine 5'-phosphate, at 2.6 A resolution and carried out autodocking analysis with D-Dopa. The structure of SULT1A3 enzyme-ligand complex clearly showed that residue Glu146 can form electrostatic interaction with dopamine and may play a pivotal role in the stereoselectivity and sulfating activity. On the other hand, residue Asp86 appeared to be critical to the Mn2+-stimulation of the Dopa/tyrosine-sulfating activity of SULT1A3, in addition to a supporting role in the stereoselectivity and sulfating activity.

Disease

Known diseases associated with this structure: Alzheimer disease-4 OMIM:[600759], Cardiomyopathy, dilated, 1V OMIM:[600759]

About this Structure

2A3R is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human sulfotransferase SULT1A3 in complex with dopamine and 3'-phosphoadenosine 5'-phosphate., Lu JH, Li HT, Liu MC, Zhang JP, Li M, An XM, Chang WR, Biochem Biophys Res Commun. 2005 Sep 23;335(2):417-23. PMID:16083857

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