1h6h
From Proteopedia
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==Overview== | ==Overview== | ||
| - | More than 50 human proteins with a wide range of functions have a 120, residue phosphoinositide binding module known as the PX domain. The 1.7 A, X-ray crystal structure of the PX domain from the p40(phox) subunit of, NADPH oxidase bound to PtdIns(3)P shows that the PX domain embraces the, 3-phosphate on one side of a water-filled, positively charged pocket and, reveals how 3-phosphoinositide specificity is achieved. A chronic, granulomatous disease (CGD)-associated mutation in the p47(phox) PX domain, that abrogates PtdIns(3)P binding maps to a conserved Arg that does not, directly interact with the phosphoinositide but instead appears to, stabilize a critical lipid binding loop. The SH3 domain present in the, full-length protein does not affect soluble PtdIns(3)P binding to the, | + | More than 50 human proteins with a wide range of functions have a 120, residue phosphoinositide binding module known as the PX domain. The 1.7 A, X-ray crystal structure of the PX domain from the p40(phox) subunit of, NADPH oxidase bound to PtdIns(3)P shows that the PX domain embraces the, 3-phosphate on one side of a water-filled, positively charged pocket and, reveals how 3-phosphoinositide specificity is achieved. A chronic, granulomatous disease (CGD)-associated mutation in the p47(phox) PX domain, that abrogates PtdIns(3)P binding maps to a conserved Arg that does not, directly interact with the phosphoinositide but instead appears to, stabilize a critical lipid binding loop. The SH3 domain present in the, full-length protein does not affect soluble PtdIns(3)P binding to the, p40(phox) PX domain. |
==About this Structure== | ==About this Structure== | ||
| - | 1H6H is a | + | 1H6H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with PIB and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: PIB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H6H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: px domain]] | [[Category: px domain]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:19:13 2007'' |
Revision as of 11:13, 5 November 2007
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STRUCTURE OF THE PX DOMAIN FROM P40PHOX BOUND TO PHOSPHATIDYLINOSITOL 3-PHOSPHATE
Overview
More than 50 human proteins with a wide range of functions have a 120, residue phosphoinositide binding module known as the PX domain. The 1.7 A, X-ray crystal structure of the PX domain from the p40(phox) subunit of, NADPH oxidase bound to PtdIns(3)P shows that the PX domain embraces the, 3-phosphate on one side of a water-filled, positively charged pocket and, reveals how 3-phosphoinositide specificity is achieved. A chronic, granulomatous disease (CGD)-associated mutation in the p47(phox) PX domain, that abrogates PtdIns(3)P binding maps to a conserved Arg that does not, directly interact with the phosphoinositide but instead appears to, stabilize a critical lipid binding loop. The SH3 domain present in the, full-length protein does not affect soluble PtdIns(3)P binding to the, p40(phox) PX domain.
About this Structure
1H6H is a Single protein structure of sequence from Homo sapiens with PIB and GOL as ligands. Structure known Active Site: PIB. Full crystallographic information is available from OCA.
Reference
The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate., Bravo J, Karathanassis D, Pacold CM, Pacold ME, Ellson CD, Anderson KE, Butler PJ, Lavenir I, Perisic O, Hawkins PT, Stephens L, Williams RL, Mol Cell. 2001 Oct;8(4):829-39. PMID:11684018
Page seeded by OCA on Mon Nov 5 13:19:13 2007
Categories: Homo sapiens | Single protein | Bravo, J. | Karathanassis, D. | Pacold, M. | Perisic, O. | Williams, R.L. | GOL | PIB | Px domain
