2a5d

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[[Image:2a5d.gif|left|200px]]<br /><applet load="2a5d" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2a5d.gif|left|200px]]
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caption="2a5d, resolution 1.80&Aring;" />
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'''Structural basis for the activation of cholera toxin by human ARF6-GTP'''<br />
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{{Structure
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|PDB= 2a5d |SIZE=350|CAPTION= <scene name='initialview01'>2a5d</scene>, resolution 1.80&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36]
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|GENE= ARF6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ctxA, toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
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}}
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'''Structural basis for the activation of cholera toxin by human ARF6-GTP'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2A5D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=GTP:'>GTP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5D OCA].
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2A5D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5D OCA].
==Reference==
==Reference==
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Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16099990 16099990]
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Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16099990 16099990]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: NAD(+)--diphthamide ADP-ribosyltransferase]]
[[Category: NAD(+)--diphthamide ADP-ribosyltransferase]]
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[[Category: protein transport/transferase]]
[[Category: protein transport/transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:45:33 2008''

Revision as of 13:45, 20 March 2008

Image:2a5d.gif


PDB ID 2a5d

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands: , , and
Gene: ARF6 (Homo sapiens), ctxA, toxA (Vibrio cholerae)
Activity: NAD(+)--diphthamide ADP-ribosyltransferase, with EC number 2.4.2.36
Coordinates: save as pdb, mmCIF, xml



Structural basis for the activation of cholera toxin by human ARF6-GTP


Overview

The Vibrio cholerae bacterium causes devastating diarrhea when it infects the human intestine. The key event is adenosine diphosphate (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by the cholera toxin A1 subunit (CTA1). This reaction is allosterically activated by human ADP-ribosylation factors (ARFs), a family of essential and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP (guanosine triphosphate) complex reveal that binding of the human activator elicits dramatic changes in CTA1 loop regions that allow nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of normal cellular protein partners, which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs.

About this Structure

2A5D is a Protein complex structure of sequences from Homo sapiens and Vibrio cholerae. Full crystallographic information is available from OCA.

Reference

Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:16099990

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