2a5r
From Proteopedia
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| - | [[Image:2a5r.gif|left|200px]] | + | [[Image:2a5r.gif|left|200px]] |
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| - | '''Complex of tetra-(4-n-methylpyridyl) porphin with monomeric parallel-stranded DNA tetraplex, snap-back 3+1 3' G-tetrad, single-residue chain reversal loops, GAG triad in the context of GAAG diagonal loop, C-MYC promoter, NMR, 6 struct.''' | + | {{Structure |
| + | |PDB= 2a5r |SIZE=350|CAPTION= <scene name='initialview01'>2a5r</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=POH:5,10,15,20-TETRA-(4-N-METHYLPYRIDYL) PORPHINE'>POH</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Complex of tetra-(4-n-methylpyridyl) porphin with monomeric parallel-stranded DNA tetraplex, snap-back 3+1 3' G-tetrad, single-residue chain reversal loops, GAG triad in the context of GAAG diagonal loop, C-MYC promoter, NMR, 6 struct.''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2A5R is a [ | + | 2A5R is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5R OCA]. |
==Reference== | ==Reference== | ||
| - | Small-molecule interaction with a five-guanine-tract G-quadruplex structure from the human MYC promoter., Phan AT, Kuryavyi V, Gaw HY, Patel DJ, Nat Chem Biol. 2005 Aug;1(3):167-73. Epub 2005 Jul 17. PMID:[http:// | + | Small-molecule interaction with a five-guanine-tract G-quadruplex structure from the human MYC promoter., Phan AT, Kuryavyi V, Gaw HY, Patel DJ, Nat Chem Biol. 2005 Aug;1(3):167-73. Epub 2005 Jul 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16408022 16408022] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: monomeric parallel-stranded quadruplex; c-myc promoter 3+1 g-tetrad; single nucleotide chain reversal loop; gag triad; gaag loop]] | [[Category: monomeric parallel-stranded quadruplex; c-myc promoter 3+1 g-tetrad; single nucleotide chain reversal loop; gag triad; gaag loop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:45:41 2008'' |
Revision as of 13:45, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Complex of tetra-(4-n-methylpyridyl) porphin with monomeric parallel-stranded DNA tetraplex, snap-back 3+1 3' G-tetrad, single-residue chain reversal loops, GAG triad in the context of GAAG diagonal loop, C-MYC promoter, NMR, 6 struct.
Overview
It has been widely accepted that DNA can adopt other biologically relevant structures beside the Watson-Crick double helix. One recent important example is the guanine-quadruplex (G-quadruplex) structure formed by guanine tracts found in the MYC (or c-myc) promoter region, which regulates the transcription of the MYC oncogene. Stabilization of this G-quadruplex by ligands, such as the cationic porphyrin TMPyP4, decreases the transcriptional level of MYC. Here, we report the first structure of a DNA fragment containing five guanine tracts from this region. An unusual G-quadruplex fold, which was derived from NMR restraints using unambiguous model-independent resonance assignment approaches, involves a core of three stacked guanine tetrads formed by four parallel guanine tracts with all anti guanines and a snapback 3'-end syn guanine. We have determined the structure of the complex formed between this G-quadruplex and TMPyP4. This structural information, combined with details of small-molecule interaction, provides a platform for the design of anticancer drugs targeting multi-guanine-tract sequences that are found in the MYC and other oncogenic promoters, as well as in telomeres.
About this Structure
2A5R is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Small-molecule interaction with a five-guanine-tract G-quadruplex structure from the human MYC promoter., Phan AT, Kuryavyi V, Gaw HY, Patel DJ, Nat Chem Biol. 2005 Aug;1(3):167-73. Epub 2005 Jul 17. PMID:16408022
Page seeded by OCA on Thu Mar 20 15:45:41 2008
