2a5m
From Proteopedia
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| - | [[Image:2a5m.gif|left|200px]] | + | [[Image:2a5m.gif|left|200px]] |
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| - | '''NMR structure of murine gamma-S crystallin from joint refinement with SAXS data''' | + | {{Structure |
| + | |PDB= 2a5m |SIZE=350|CAPTION= <scene name='initialview01'>2a5m</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Crygs ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | }} | ||
| + | |||
| + | '''NMR structure of murine gamma-S crystallin from joint refinement with SAXS data''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2A5M is a [ | + | 2A5M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5M OCA]. |
==Reference== | ==Reference== | ||
| - | Refinement of multidomain protein structures by combination of solution small-angle X-ray scattering and NMR data., Grishaev A, Wu J, Trewhella J, Bax A, J Am Chem Soc. 2005 Nov 30;127(47):16621-8. PMID:[http:// | + | Refinement of multidomain protein structures by combination of solution small-angle X-ray scattering and NMR data., Grishaev A, Wu J, Trewhella J, Bax A, J Am Chem Soc. 2005 Nov 30;127(47):16621-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16305251 16305251] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rdc]] | [[Category: rdc]] | ||
[[Category: residual dipolar coupling]] | [[Category: residual dipolar coupling]] | ||
| - | [[Category: | + | [[Category: sax]] |
[[Category: small-angle x-ray scattering]] | [[Category: small-angle x-ray scattering]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:45:43 2008'' |
Revision as of 13:45, 20 March 2008
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| Gene: | Crygs (Mus musculus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR structure of murine gamma-S crystallin from joint refinement with SAXS data
Overview
Determination of the 3D structures of multidomain proteins by solution NMR methods presents a number of unique challenges related to their larger molecular size and the usual scarcity of constraints at the interdomain interface, often resulting in a decrease in structural accuracy. In this respect, experimental information from small-angle scattering of X-ray radiation in solution (SAXS) presents a suitable complement to the NMR data, as it provides an independent constraint on the overall molecular shape. A computational procedure is described that allows incorporation of such SAXS data into the mainstream high-resolution macromolecular structure refinement. The method is illustrated for a two-domain 177-amino-acid protein, gammaS crystallin, using an experimental SAXS data set fitted at resolutions from approximately 200 A to approximately 30 A. Inclusion of these data during structure refinement decreases the backbone coordinate root-mean-square difference between the derived model and the high-resolution crystal structure of a 54% homologous gammaB crystallin from 1.96 +/- 0.07 A to 1.31 +/- 0.04 A. Combining SAXS data with NMR restraints can be accomplished at a moderate computational expense and is expected to become useful for multidomain proteins, multimeric assemblies, and tight macromolecular complexes.
About this Structure
2A5M is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Refinement of multidomain protein structures by combination of solution small-angle X-ray scattering and NMR data., Grishaev A, Wu J, Trewhella J, Bax A, J Am Chem Soc. 2005 Nov 30;127(47):16621-8. PMID:16305251
Page seeded by OCA on Thu Mar 20 15:45:43 2008
