1j9i
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1j9i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J9I FirstGlance]. <br> | <table><tr><td colspan='2'>[[1j9i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J9I FirstGlance]. <br> | ||
| - | </td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NU1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])</td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NU1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])</td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j9i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j9i RCSB], [http://www.ebi.ac.uk/pdbsum/1j9i PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j9i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j9i RCSB], [http://www.ebi.ac.uk/pdbsum/1j9i PDBsum]</span></td></tr> |
| - | <table> | + | </table> |
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/TERS_LAMBD TERS_LAMBD]] Component of the terminase that processes and encapsidates viral genomes during virion assembly. The terminase is composed of two small and one large subunits. To initiate packaging, it binds a specific sequence called cos, at the junction of adjacent viral genomes in the concatemeric DNA substrate. Next, in a reaction stimulated by the presence of proheads and ATP but not requiring ATP hydrolysis, the terminase creates two nicks 12bp appart at the cos site, one on each stand. Terminase then separates the cohesive ends in a reaction requiring ATP hydrolysis. The heterotrimer remains bound to the left end of the genome to be packaged, forming a stable DNA-protein complex known as complex I. In a reaction facilitated by a viral assembly catalyst, gpFI, complex I binds a prohead, a preformed head shell precursor, to form complex II. In another packaging reaction requiring ATP hydrolysis, the DNA is translocated into the prohead until the next cos site on the concatemer reaches the packaging complex. At this time the downstream cos site is cut and the heterotrimer undocks from the DNA-filled head to remain bound to the left end of concatemer's next genome. The new heterotrimer-DNA complex I binds another prohead to continue the processive, polarized packaging of viral genomes. The terminase is dependent upon host integration host factor (ihfA/ihfB) for these activities.<ref>PMID:2989542</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Enterobacteria phage lambda]] | [[Category: Enterobacteria phage lambda]] | ||
| - | [[Category: Beer, T De | + | [[Category: Beer, T De]] |
| - | [[Category: Berton, N | + | [[Category: Berton, N]] |
| - | [[Category: Catalano, C | + | [[Category: Catalano, C]] |
| - | [[Category: Duffy, C | + | [[Category: Duffy, C]] |
| - | [[Category: Feiss, M | + | [[Category: Feiss, M]] |
| - | [[Category: Maes, L | + | [[Category: Maes, L]] |
| - | [[Category: Meyer, J | + | [[Category: Meyer, J]] |
| - | [[Category: Ortega, M | + | [[Category: Ortega, M]] |
| - | [[Category: Overduin, M | + | [[Category: Overduin, M]] |
| - | [[Category: Sippy, J | + | [[Category: Sippy, J]] |
| - | [[Category: Yang, Q | + | [[Category: Yang, Q]] |
[[Category: Dna binding domain]] | [[Category: Dna binding domain]] | ||
[[Category: Homodimer]] | [[Category: Homodimer]] | ||
Revision as of 23:22, 24 December 2014
STRUCTURE OF THE DNA BINDING DOMAIN OF THE GPNU1 SUBUNIT OF LAMBDA TERMINASE
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